000096021 001__ 96021
000096021 005__ 20201029165924.0
000096021 0247_ $$2doi$$a10.17235/reed.2018.5288/2017
000096021 0248_ $$2sideral$$a112466
000096021 037__ $$aART-2019-112466
000096021 041__ $$aeng
000096021 100__ $$0(orcid)0000-0001-7559-1763$$aAndrés Lasheras, Sara
000096021 245__ $$aIncidence and characterization of Clostridium difficile in a secondary care hospital in Spain
000096021 260__ $$c2019
000096021 5060_ $$aAccess copy available to the general public$$fUnrestricted
000096021 5203_ $$aINTRODUCTION: Clostridium difficile (C. difficile) is a major nosocomial infectious agent in hospitals. Previous studies have addressed the high proportion of infection episodes that are overlooked in health care facilities. OBJECTIVE: the main aim of this study was to characterize C. difficile clinical cases that occurred in a secondary care hospital during a five-month period. MATERIAL AND METHODS: for this purpose, a total of 137 stool samples from the same number of patients with diarrhea were analyzed for the presence of C. difficile by culture techniques. An enzyme immunoassay (EIA) test for the detection of C. difficile and its toxins was also used in 50 cases (36.5%) for diagnostic purposes.
RESULTS: a total of 14 (10.2%) C. difficile isolates were obtained, of which nine (64.3%) were toxigenic. A mean incidence of 3.2 episodes of C. difficile infections (CDI) per 10, 000 patients-days was estimated for the study period. Around 56% of the CDI cases were determined as hospital-acquired, whereas 44% originated in the community. Among these, only two episodes (22.2%) were detected in the hospital by the EIA test, which indicated that the hospital CDI detection protocol needed to be revised. One unusual C. difficile isolate was negative for all toxin genes examined and also for the non-toxigenic strain assay, which highlights the need to perform genome sequencing to study its pathogenicity locus insertion site organization. A stable metronidazole-resistant C. difficile strain and three strains showing multidrug resistance were detected in this study, suggesting that C. difficile antimicrobial susceptibility surveillance programs should be established in this health-care facility.
000096021 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000096021 590__ $$a1.591$$b2019
000096021 591__ $$aGASTROENTEROLOGY & HEPATOLOGY$$b81 / 88 = 0.92$$c2019$$dQ4$$eT3
000096021 592__ $$a0.383$$b2019
000096021 593__ $$aMedicine (miscellaneous)$$c2019$$dQ3
000096021 593__ $$aGastroenterology$$c2019$$dQ3
000096021 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000096021 700__ $$0(orcid)0000-0001-6016-4726$$aMartín Burriel, Inma$$uUniversidad de Zaragoza
000096021 700__ $$aAspiroz, Carmen
000096021 700__ $$0(orcid)0000-0001-5442-7702$$aMainar Jaime, Raul Carlos$$uUniversidad de Zaragoza
000096021 700__ $$aRobres, Pilar
000096021 700__ $$0(orcid)0000-0002-4213-2904$$aSevilla, Eloísa$$uUniversidad de Zaragoza
000096021 700__ $$aKuijper, Ed
000096021 700__ $$aChirino Trejo, Manuel
000096021 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000096021 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000096021 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000096021 773__ $$g111, 5 (2019), 338-344$$pRev. esp. enferm. dig.$$tREVISTA ESPANOLA DE ENFERMEDADES DIGESTIVAS$$x1130-0108
000096021 8564_ $$s615969$$uhttps://zaguan.unizar.es/record/96021/files/texto_completo.pdf$$yVersión publicada
000096021 8564_ $$s24782$$uhttps://zaguan.unizar.es/record/96021/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000096021 909CO $$ooai:zaguan.unizar.es:96021$$particulos$$pdriver
000096021 951__ $$a2020-10-29-14:06:21
000096021 980__ $$aARTICLE