000096088 001__ 96088
000096088 005__ 20210902121835.0
000096088 0247_ $$2doi$$a10.1038/s41467-020-18954-z
000096088 0248_ $$2sideral$$a120752
000096088 037__ $$aART-2020-120752
000096088 041__ $$aeng
000096088 100__ $$aValle, S.
000096088 245__ $$aExploiting oxidative phosphorylation to promote the stem and immunoevasive properties of pancreatic cancer stem cells
000096088 260__ $$c2020
000096088 5060_ $$aAccess copy available to the general public$$fUnrestricted
000096088 5203_ $$aPancreatic ductal adenocarcinoma (PDAC), the fourth leading cause of cancer death, has a 5-year survival rate of approximately 7–9%. The ineffectiveness of anti-PDAC therapies is believed to be due to the existence of a subpopulation of tumor cells known as cancer stem cells (CSCs), which are functionally plastic, and have exclusive tumorigenic, chemoresistant and metastatic capacities. Herein, we describe a 2D in vitro system for long-term enrichment of pancreatic CSCs that is amenable to biological and CSC-specific studies. By changing the carbon source from glucose to galactose in vitro, we force PDAC cells to utilize OXPHOS, resulting in enrichment of CSCs defined by increased CSC biomarker and pluripotency gene expression, greater tumorigenic potential, induced but reversible quiescence, increased OXPHOS activity, enhanced invasiveness, and upregulated immune evasion properties. This CSC enrichment method can facilitate the discovery of new CSC-specific hallmarks for future development into targets for PDAC-based therapies.
000096088 536__ $$9info:eu-repo/grantAgreement/ES/FEDER/Una manera de hacer Europa$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI16-00789$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI18-00757$$9info:eu-repo/grantAgreement/ES/ISCIII/FIS/PI18-01347$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2012-12104
000096088 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000096088 590__ $$a14.919$$b2020
000096088 591__ $$aMULTIDISCIPLINARY SCIENCES$$b4 / 73 = 0.055$$c2020$$dQ1$$eT1
000096088 592__ $$a5.559$$b2020
000096088 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2020$$dQ1
000096088 593__ $$aPhysics and Astronomy (miscellaneous)$$c2020$$dQ1
000096088 593__ $$aChemistry (miscellaneous)$$c2020$$dQ1
000096088 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000096088 700__ $$aAlcalá, S.
000096088 700__ $$aMartin-Hijano, L.
000096088 700__ $$aCabezas-Sáinz, P.
000096088 700__ $$aNavarro, D.
000096088 700__ $$aMuñoz, E.R.
000096088 700__ $$aYuste, L.
000096088 700__ $$aTiwary, K.
000096088 700__ $$aWalter, K.
000096088 700__ $$aRuiz-Cañas, L.
000096088 700__ $$aAlonso-Nocelo, M.
000096088 700__ $$aRubiolo, J.A.
000096088 700__ $$aGonzález-Arnay, E.
000096088 700__ $$aHeeschen, C.
000096088 700__ $$aGarcia-Bermejo, L.
000096088 700__ $$aHermann, P.C.
000096088 700__ $$aSánchez, L.
000096088 700__ $$0(orcid)0000-0002-8624-8757$$aSancho, P.
000096088 700__ $$aFernández-Moreno, M.Á.
000096088 700__ $$aSainz, B.
000096088 700__ $$a, Jr.
000096088 773__ $$g11, 1 (2020), 5265 [19 pp]$$pNATURE COMMUNICATIONS$$tNature Communications$$x2041-1723
000096088 8564_ $$s1327143$$uhttps://zaguan.unizar.es/record/96088/files/texto_completo.pdf$$yVersión publicada
000096088 8564_ $$s61022$$uhttps://zaguan.unizar.es/record/96088/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
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000096088 951__ $$a2021-09-02-10:20:10
000096088 980__ $$aARTICLE