doi:10.3390/ijms21197331engAbuobeid, R.Herrera-Marcos, L.Navarro, M.A.Arnal, C.Martínez-Beamonte, R.Surra, J.Osada, J.Dietary erythrodiol modifies hepatic transcriptome in mice in a sex and dose-dependent wayART-2020-120761Erythrodiol is a terpenic compound found in a large number of plants. To test the hypotheses that its long-term administration may influence hepatic transcriptome and this could be influenced by the presence of APOA1-containing high-density lipoproteins (HDL), Western diets containing 0.01% of erythrodiol (10 mg/kg dose) were provided to Apoe-and Apoa1-deficient mice. Hepatic RNA-sequencing was carried out in male Apoe-deficient mice fed purified Western diets differing in the erythrodiol content. The administration of this compound significantly up-regulated 68 and down-regulated 124 genes at the level of 2-fold change. These genes belonged to detoxification processes, protein metabolism and nucleic acid related metabolites. Gene expression changes of 21 selected transcripts were verified by RT-qPCR. Ccl19-ps2, Cyp2b10, Rbm14-rbm4, Sec61g, Tmem81, Prtn3, Amy2a5, Cyp2b9 and Mup1 showed significant changes by erythrodiol administration. When Cyp2b10, Dmbt1, Cyp2b13, Prtn3 and Cyp2b9 were analyzed in female Apoe-deficient mice, no change was observed. Likewise, no significant variation was observed in Apoa1-or in Apoe-deficient mice receiving doses ranging from 0.5 to 5 mg/kg erythrodiol. Our results give evidence that erythrodiol exerts a hepatic transcriptional role, but this is selective in terms of sex and requires a threshold dose. Furthermore, it requires an APOA1-containing HDL.2020http://zaguan.unizar.es/record/9611510.3390/ijms21197331http://zaguan.unizar.es/record/96115oai:zaguan.unizar.es:96115info:eu-repo/grantAgreement/ES/CIBERObn/CB06-03-1012info:eu-repo/grantAgreement/ES/DGA/FSEinfo:eu-repo/grantAgreement/ES/DGA-FSE/B16-20Rinfo:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-RInternational Journal of Molecular Sciences 21, 19 (2020), 7331 [21 pp]byhttp://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccess