96115 20240123082600.0 doi 10.3390/ijms21197331 sideral 120761 ART-2020-120761 eng Abuobeid, R. Dietary erythrodiol modifies hepatic transcriptome in mice in a sex and dose-dependent way 2020 Access copy available to the general public Unrestricted Erythrodiol is a terpenic compound found in a large number of plants. To test the hypotheses that its long-term administration may influence hepatic transcriptome and this could be influenced by the presence of APOA1-containing high-density lipoproteins (HDL), Western diets containing 0.01% of erythrodiol (10 mg/kg dose) were provided to Apoe-and Apoa1-deficient mice. Hepatic RNA-sequencing was carried out in male Apoe-deficient mice fed purified Western diets differing in the erythrodiol content. The administration of this compound significantly up-regulated 68 and down-regulated 124 genes at the level of 2-fold change. These genes belonged to detoxification processes, protein metabolism and nucleic acid related metabolites. Gene expression changes of 21 selected transcripts were verified by RT-qPCR. Ccl19-ps2, Cyp2b10, Rbm14-rbm4, Sec61g, Tmem81, Prtn3, Amy2a5, Cyp2b9 and Mup1 showed significant changes by erythrodiol administration. When Cyp2b10, Dmbt1, Cyp2b13, Prtn3 and Cyp2b9 were analyzed in female Apoe-deficient mice, no change was observed. Likewise, no significant variation was observed in Apoa1-or in Apoe-deficient mice receiving doses ranging from 0.5 to 5 mg/kg erythrodiol. Our results give evidence that erythrodiol exerts a hepatic transcriptional role, but this is selective in terms of sex and requires a threshold dose. Furthermore, it requires an APOA1-containing HDL. info:eu-repo/grantAgreement/ES/CIBERObn/CB06-03-1012 info:eu-repo/grantAgreement/ES/DGA/FSE info:eu-repo/grantAgreement/ES/DGA-FSE/B16-20R info:eu-repo/grantAgreement/ES/MICINN-ISCIIII-FEDER/PID2019-104915RB-I00 info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.923 2020 BIOCHEMISTRY & MOLECULAR BIOLOGY 67 / 296 = 0.226 2020 Q1 T1 CHEMISTRY, MULTIDISCIPLINARY 49 / 178 = 0.275 2020 Q2 T1 1.455 2020 Catalysis 2020 Q1 Computer Science Applications 2020 Q1 Inorganic Chemistry 2020 Q1 Spectroscopy 2020 Q1 Molecular Biology 2020 Q1 Organic Chemistry 2020 Q1 Physical and Theoretical Chemistry 2020 Q1 Medicine (miscellaneous) 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Herrera-Marcos, L. (orcid)0000-0002-4665-9674 Navarro, M.A. Universidad de Zaragoza (orcid)0000-0002-0108-1004 Arnal, C. Universidad de Zaragoza (orcid)0000-0003-1197-4276 Martínez-Beamonte, R. Universidad de Zaragoza (orcid)0000-0002-8100-5596 Surra, J. Universidad de Zaragoza (orcid)0000-0002-5841-0462 Osada, J. Universidad de Zaragoza (orcid)0000-0002-8251-8457 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 21, 19 (2020), 7331 [21 pp] Int. j. mol. sci. International Journal of Molecular Sciences 1661-6596 712979 http://zaguan.unizar.es/record/96115/files/texto_completo.pdf Versión publicada 504629 http://zaguan.unizar.es/record/96115/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:96115 articulos driver 2024-01-23-08:21:28 ARTICLE