000096163 001__ 96163
000096163 005__ 20230622083313.0
000096163 0247_ $$2doi$$a10.3390/tropicalmed5030149
000096163 0248_ $$2sideral$$a120726
000096163 037__ $$aART-2020-120726
000096163 041__ $$aeng
000096163 100__ $$aFlores, B.
000096163 245__ $$aDetection of Pathogenic Leptospires in Water and Soil in Areas Endemic to Leptospirosis in Nicaragua
000096163 260__ $$c2020
000096163 5060_ $$aAccess copy available to the general public$$fUnrestricted
000096163 5203_ $$aIn Nicaragua, there are ideal environmental conditions for leptospirosis. The objective of this investigation was to detect pathogenic and saprophytic leptospires in water and soil samples from leptospirosis-endemic areas in Nicaragua. Seventy-eight water and 42 soil samples were collected from houses and rivers close to confirmed human cases.Leptospiraspp was isolated in Ellinghausen-McCullough-Johnson-Harris (EMJH) culture medium with 5-fluororacil and positive samples were analyzed through PCR for theLipL32gene, specific for pathogenic leptospires (P1 clade). There were 73 positive cultures from 120 samples, however only six of these (5% of all collected samples) were confirmed to be pathogenic, based on the presence of theLipL32gene (P1 clade). Of these six pathogenic isolates, four were from Leon and two from Chinandega. Four pathogenic isolates were obtained from water and two from soil. This study proved the contamination of water and soil with pathogenic leptospires, which represents a potential risk for public health.
000096163 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000096163 592__ $$a1.042$$b2020
000096163 593__ $$aPublic Health, Environmental and Occupational Health$$c2020$$dQ1
000096163 593__ $$aInfectious Diseases$$c2020$$dQ1
000096163 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000096163 700__ $$aEscobar, K.
000096163 700__ $$0(orcid)0000-0001-5422-1122$$aMuzquiz, J.L.$$uUniversidad de Zaragoza
000096163 700__ $$aSheleby-Elias, J.
000096163 700__ $$aMora, B.
000096163 700__ $$aRoque, E.
000096163 700__ $$aTorres, D.
000096163 700__ $$aChavez, A.
000096163 700__ $$aJiron, W.
000096163 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000096163 773__ $$g5, 3 (2020), 149 [10 pp]$$tTROPICAL MEDICINE AND INFECTIOUS DISEASE$$x2414-6366
000096163 8564_ $$s571199$$uhttps://zaguan.unizar.es/record/96163/files/texto_completo.pdf$$yVersión publicada
000096163 8564_ $$s500520$$uhttps://zaguan.unizar.es/record/96163/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000096163 909CO $$ooai:zaguan.unizar.es:96163$$particulos$$pdriver
000096163 951__ $$a2023-06-21-15:00:58
000096163 980__ $$aARTICLE