96185 20210902121845.0 doi 10.1186/s12951-020-00701-7 sideral 120745 ART-2020-120745 eng Sancho-Albero, M. Universidad de Zaragoza (orcid)0000-0001-8762-5457 Isolation of exosomes from whole blood by a new microfluidic device: proof of concept application in the diagnosis and monitoring of pancreatic cancer 2020 Access copy available to the general public Unrestricted Background: Exosomes are endocytic-extracellular vesicles with a diameter around 100 nm that play an essential role on the communication between cells. In fact, they have been proposed as candidates for the diagnosis and the monitoring of different pathologies (such as Parkinson, Alzheimer, diabetes, cardiac damage, infection diseases or cancer). Results: In this study, magnetic nanoparticles (Fe3O4NPs) were successfully functionalized with an exosome-binding antibody (anti-CD9) to mediate the magnetic capture in a microdevice. This was carried out under flow in a 1.6 mm (outer diameter) microchannel whose wall was in contact with a set of NdFeB permanent magnets, giving a high magnetic field across the channel diameter that allowed exosome separation with a high yield. To show the usefulness of the method, the direct capture of exosomes from whole blood of patients with pancreatic cancer (PC) was performed, as a proof of concept. The captured exosomes were then subjected to analysis of CA19-9, a protein often used to monitor PC patients. Conclusions: Here, we describe a new microfluidic device and the procedure for the isolation of exosomes from whole blood, without any need of previous isolation steps, thereby facilitating translation to the clinic. The results show that, for the cases analyzed, the evaluation of CA19-9 in exosomes was highly sensitive, compared to serum samples. info:eu-repo/grantAgreement/ES/UZ-DGA/T57-17R-P info:eu-repo/grantAgreement/EUR/ERC-2013-CoG-614715 info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 742684-CADENCE info:eu-repo/grantAgreement/ES/ISCIII-IIS/MS19-00092 info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 10.435 2020 NANOSCIENCE & NANOTECHNOLOGY 19 / 106 = 0.179 2020 Q1 T1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY 8 / 159 = 0.05 2020 Q1 T1 1.629 2020 Applied Microbiology and Biotechnology 2020 Q1 Bioengineering 2020 Q1 Biomedical Engineering 2020 Q1 Pharmaceutical Science 2020 Q1 Molecular Medicine 2020 Q1 Nanoscience and Nanotechnology 2020 Q1 Medicine (miscellaneous) 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sebastián, V. Universidad de Zaragoza (orcid)0000-0002-6873-5244 Sesé, J. Universidad de Zaragoza (orcid)0000-0002-7742-9329 Pazo-Cid, R. Universidad de Zaragoza (orcid)0000-0002-8026-7391 Mendoza, G. Universidad de Zaragoza (orcid)0000-0003-2293-363X Arruebo, M. Universidad de Zaragoza (orcid)0000-0003-3165-0156 Martín-Duque, P. (orcid)0000-0003-4160-9720 Santamaría, J. Universidad de Zaragoza (orcid)0000-0002-8701-9745 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 2003 395 Universidad de Zaragoza Dpto. Física Materia Condensa. Área Física Materia Condensada 18 (2020), 150 [15 pp] J. nanobiotechnol. Journal of Nanobiotechnology 1477-3155 3844051 http://zaguan.unizar.es/record/96185/files/texto_completo.pdf Versión publicada 36837 http://zaguan.unizar.es/record/96185/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:96185 articulos driver 2021-09-02-10:26:41 ARTICLE