000096812 001__ 96812
000096812 005__ 20201124104756.0
000096812 0247_ $$2doi$$a10.3390/ma12142218
000096812 0248_ $$2sideral$$a113171
000096812 037__ $$aART-2019-113171
000096812 041__ $$aeng
000096812 100__ $$aRuiz, A.
000096812 245__ $$aUnderstanding the Influence of a Bifunctional Polyethylene Glycol Derivative in Protein Corona Formation around Iron Oxide Nanoparticles
000096812 260__ $$c2019
000096812 5060_ $$aAccess copy available to the general public$$fUnrestricted
000096812 5203_ $$aSuperparamagnetic iron oxide nanoparticles are one of the most prominent agents used in theranostic applications, with MRI imaging the main application assessed. The biomolecular interface formed on the surface of a nanoparticle in a biological medium determines its behaviour in vitro and in vivo. In this study, we have compared the formation of the protein corona on highly monodisperse iron oxide nanoparticles with two different coatings, dimercaptosuccinic acid (DMSA), and after conjugation, with a bifunctional polyethylene glycol (PEG)-derived molecule (2000 Da) in the presence of Wistar rat plasma. The protein fingerprints around the nanoparticles were analysed in an extensive proteomic study. The results presented in this work indicate that the composition of the protein corona is very difficult to predict. Proteins from different functional categories cell components, lipoproteins, complement, coagulation, immunoglobulins, enzymes and transport proteins were identified in all samples with very small variability. Although both types of nanoparticles have similar amounts of bonded proteins, very slight differences in the composition of the corona might explain the variation observed in the uptake and biotransformation of these nanoparticles in Caco-2 and RAW 264.7 cells. Cytotoxicity was also studied using a standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Controlling nanoparticles' reactivity to the biological environment by deciding on its surface functionalization may suggest new routes in the control of the biodistribution, biodegradation and clearance of multifunctional nanomedicines.
000096812 536__ $$9info:eu-repo/grantAgreement/ES/CSIC-CITMA/B01CU2009$$9info:eu-repo/grantAgreement/ES/CSIC-CITMA/ICMM-2011-2014$$9info:eu-repo/grantAgreement/ES/CSIC-CITMA/MAT2017-88148R$$9info:eu-repo/grantAgreement/EC/FP7/612338/EU/DNA-TRAP Delivery of Nucleic Acid-Based Therapeutics for the TReatment of Antibiotic-Resistant Pathogens/DNA-TRAP
000096812 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000096812 590__ $$a3.057$$b2019
000096812 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b132 / 314 = 0.42$$c2019$$dQ2$$eT2
000096812 592__ $$a0.647$$b2019
000096812 593__ $$aMaterials Science (miscellaneous)$$c2019$$dQ2
000096812 593__ $$aCondensed Matter Physics$$c2019$$dQ2
000096812 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000096812 700__ $$aAlpizar, A.
000096812 700__ $$0(orcid)0000-0003-4516-8694$$aBeola, L.
000096812 700__ $$aRubio, C.
000096812 700__ $$aGavilan, H.
000096812 700__ $$aMarciello, M.
000096812 700__ $$aRodriguez-Ramiro, I.
000096812 700__ $$aCiordia, S.
000096812 700__ $$aMorris, C.J.
000096812 700__ $$aMorales, M.D.
000096812 773__ $$g12, 14 (2019), 2218 [18 pp]$$pMATERIALS$$tMATERIALS$$x1996-1944
000096812 8564_ $$s752219$$uhttps://zaguan.unizar.es/record/96812/files/texto_completo.pdf$$yVersión publicada
000096812 8564_ $$s473134$$uhttps://zaguan.unizar.es/record/96812/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000096812 909CO $$ooai:zaguan.unizar.es:96812$$particulos$$pdriver
000096812 951__ $$a2020-11-22-12:38:56
000096812 980__ $$aARTICLE