000096815 001__ 96815
000096815 005__ 20201124104756.0
000096815 0247_ $$2doi$$a10.1038/s41598-019-45726-7
000096815 0248_ $$2sideral$$a113195
000096815 037__ $$aART-2019-113195
000096815 041__ $$aeng
000096815 100__ $$aFranco, Aitor
000096815 245__ $$aStructural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition
000096815 260__ $$c2019
000096815 5060_ $$aAccess copy available to the general public$$fUnrestricted
000096815 5203_ $$aNucleoplasmin (NP) is a pentameric histone chaperone that regulates the condensation state of chromatin in different cellular processes. We focus here on the interaction of NP with the histone octamer, showing that NP could bind sequentially the histone components to assemble an octamer-like particle, and crosslinked octamers with high affinity. The three-dimensional reconstruction of the NP/octamer complex generated by single-particle cryoelectron microscopy, revealed that several intrinsically disordered tail domains of two NP pentamers, facing each other through their distal face, encage the histone octamer in a nucleosome-like conformation and prevent its dissociation. Formation of this complex depended on post-translational modification and exposure of the acidic tract at the tail domain of NP. Finally, NP was capable of transferring the histone octamers to DNA in vitro, assembling nucleosomes. This activity may have biological relevance for processes in which the histone octamer must be rapidly removed from or deposited onto the DNA.
000096815 536__ $$9info:eu-repo/grantAgreement/ES/AEI-FEDER/BFU2016-75983$$9info:eu-repo/grantAgreement/ES/AEI-FEDER/BFU2016-75984
000096815 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000096815 590__ $$a3.998$$b2019
000096815 591__ $$aMULTIDISCIPLINARY SCIENCES$$b17 / 71 = 0.239$$c2019$$dQ1$$eT1
000096815 592__ $$a1.341$$b2019
000096815 593__ $$aMultidisciplinary$$c2019$$dQ1
000096815 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000096815 700__ $$aArranz, Rocío
000096815 700__ $$aFernandez-Rivero, Noelia
000096815 700__ $$0(orcid)0000-0001-5702-4538$$aVelazquez-Campoy, Adrián$$uUniversidad de Zaragoza
000096815 700__ $$aMartin-Benito, Jaime
000096815 700__ $$aSegura, Joan
000096815 700__ $$aPrado, Adelina
000096815 700__ $$aValpuesta, José M.
000096815 700__ $$aMuga, Arturo
000096815 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000096815 773__ $$g9 (2019), 9487 [14 pp]$$pSci. rep.$$tScientific Reports$$x2045-2322
000096815 8564_ $$s1101410$$uhttps://zaguan.unizar.es/record/96815/files/texto_completo.pdf$$yVersión publicada
000096815 8564_ $$s205646$$uhttps://zaguan.unizar.es/record/96815/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000096815 909CO $$ooai:zaguan.unizar.es:96815$$particulos$$pdriver
000096815 951__ $$a2020-11-22-12:39:19
000096815 980__ $$aARTICLE