96815 20201124104756.0 doi 10.1038/s41598-019-45726-7 sideral 113195 ART-2019-113195 eng Franco, Aitor Structural insights into the ability of nucleoplasmin to assemble and chaperone histone octamers for DNA deposition 2019 Access copy available to the general public Unrestricted Nucleoplasmin (NP) is a pentameric histone chaperone that regulates the condensation state of chromatin in different cellular processes. We focus here on the interaction of NP with the histone octamer, showing that NP could bind sequentially the histone components to assemble an octamer-like particle, and crosslinked octamers with high affinity. The three-dimensional reconstruction of the NP/octamer complex generated by single-particle cryoelectron microscopy, revealed that several intrinsically disordered tail domains of two NP pentamers, facing each other through their distal face, encage the histone octamer in a nucleosome-like conformation and prevent its dissociation. Formation of this complex depended on post-translational modification and exposure of the acidic tract at the tail domain of NP. Finally, NP was capable of transferring the histone octamers to DNA in vitro, assembling nucleosomes. This activity may have biological relevance for processes in which the histone octamer must be rapidly removed from or deposited onto the DNA. info:eu-repo/grantAgreement/ES/AEI-FEDER/BFU2016-75983 info:eu-repo/grantAgreement/ES/AEI-FEDER/BFU2016-75984 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.998 2019 MULTIDISCIPLINARY SCIENCES 17 / 71 = 0.239 2019 Q1 T1 1.341 2019 Multidisciplinary 2019 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Arranz, Rocío Fernandez-Rivero, Noelia (orcid)0000-0001-5702-4538 Velazquez-Campoy, Adrián Universidad de Zaragoza Martin-Benito, Jaime Segura, Joan Prado, Adelina Valpuesta, José M. Muga, Arturo 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 9 (2019), 9487 [14 pp] Sci. rep. Scientific Reports 2045-2322 1101410 http://zaguan.unizar.es/record/96815/files/texto_completo.pdf Versión publicada 205646 http://zaguan.unizar.es/record/96815/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:96815 articulos driver 2020-11-22-12:39:19 ARTICLE