000097119 001__ 97119
000097119 005__ 20210902121908.0
000097119 0247_ $$2doi$$a10.3389/fpls.2020.600336
000097119 0248_ $$2sideral$$a121231
000097119 037__ $$aART-2020-121231
000097119 041__ $$aeng
000097119 100__ $$aVillar, Irene
000097119 245__ $$aA Plant Gene Encoding One-Heme and Two-Heme Hemoglobins With Extreme Reactivities Toward Diatomic Gases and Nitrite
000097119 260__ $$c2020
000097119 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097119 5203_ $$aIn plants, symbiotic hemoglobins act as carriers and buffers of O2 in nodules, whereas nonsymbiotic hemoglobins or phytoglobins (Glbs) are ubiquitous in tissues and may perform multiple, but still poorly defined, functions related to O2 and/or nitric oxide (NO). Here, we have identified a Glb gene of the model legume Medicago truncatula with unique properties. The gene, designated MtGlb1-2, generates four alternative splice forms encoding Glbs with one or two heme domains and 215–351 amino acid residues. This is more than double the size of any hemoglobin from plants or other organisms described so far. A combination of molecular, cellular, biochemical, and biophysical methods was used to characterize these novel proteins. RNA-sequencing showed that the four splice variants are expressed in plant tissues. MtGlb1-2 is transcriptionally activated by hypoxia and its expression is further enhanced by an NO source. The gene is preferentially expressed in the meristems and vascular bundles of roots and nodules. Two of the proteins, bearing one or two hemes, were characterized using mutants in the distal histidines of the hemes. The Glbs are extremely reactive toward the physiological ligands O2, NO, and nitrite. They show very high O2 affinities, NO dioxygenase activity (in the presence of O2), and nitrite reductase (NiR) activity (in the absence of O2) compared with the hemoglobins from vertebrates and other plants. We propose that these Glbs act as either NO scavengers or NO producers depending on the O2 tension in the plant tissue, being involved in the fast and fine tuning of NO concentration in the cytosol in response to sudden changes in O2 availability.
000097119 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A09-17R$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-85775--R$$9info:eu-repo/grantAgreement/ES/MINECO/RTI2018-094623-B-C22
000097119 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000097119 590__ $$a5.753$$b2020
000097119 591__ $$aPLANT SCIENCES$$b17 / 235 = 0.072$$c2020$$dQ1$$eT1
000097119 592__ $$a1.752$$b2020
000097119 593__ $$aPlant Science$$c2020$$dQ1
000097119 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097119 700__ $$aLarrainzar, Estíbaliz
000097119 700__ $$aMilazzo, Lisa
000097119 700__ $$aPérez-Rontomé, Carmen
000097119 700__ $$aRubio, Maria C.
000097119 700__ $$aSmulevich, Giulietta
000097119 700__ $$0(orcid)0000-0002-5406-3280$$aMartínez, Jesús I.$$uUniversidad de Zaragoza
000097119 700__ $$aWilson, Michael T.
000097119 700__ $$aReeder, Brandon
000097119 700__ $$aHuertas, Raul
000097119 700__ $$aAbbruzzetti, Stefania
000097119 700__ $$aUdvardi, Michael
000097119 700__ $$aBecana, Manuel.
000097119 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000097119 773__ $$g11 (2020), 600336 1-14$$pFront. plant sci.$$tFrontiers in Plant Science$$x1664-462X
000097119 8564_ $$s3520608$$uhttps://zaguan.unizar.es/record/97119/files/texto_completo.pdf$$yVersión publicada
000097119 8564_ $$s29645$$uhttps://zaguan.unizar.es/record/97119/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097119 909CO $$ooai:zaguan.unizar.es:97119$$particulos$$pdriver
000097119 951__ $$a2021-09-02-10:40:51
000097119 980__ $$aARTICLE