Minimally invasive system to reliably characterize ventricular electrophysiology from living donors

Oliván-Viguera, A. ; Pérez-Zabalza, M. (Universidad de Zaragoza) ; García-Mendívil, L. (Universidad de Zaragoza) ; Mountris, K.A. ; Orós-Rodrigo, S. ; Ramos-Marquès, E. (Universidad de Zaragoza) ; Vallejo-Gil, J.M. ; Fresneda-Roldán, P.C. ; Fañanás-Mastral, J. ; Vázquez-Sancho, M. ; Matamala-Adell, M. (Universidad de Zaragoza) ; Sorribas-Berjón, F. ; Bellido-Morales, J.A. ; Mancebón-Sierra, F.J. ; Vaca-Núñez, A.S. ; Ballester-Cuenca, C. (Universidad de Zaragoza) ; Marigil, M.Á. ; Pastor, C. (Universidad de Zaragoza) ; Ordovás, L. ; Köhler, R. ; Diez, E. ; Pueyo, E. (Universidad de Zaragoza)

Resumen: Cardiac tissue slices preserve the heterogeneous structure and multicellularity of the myocardium and allow its functional characterization. However, access to human ventricular samples is scarce. We aim to demonstrate that slices from small transmural core biopsies collected from living donors during routine cardiac surgery preserve structural and functional properties of larger myocardial specimens, allowing accurate electrophysiological characterization. In pigs, we compared left ventricular transmural core biopsies with transmural tissue blocks from the same ventricular region. In humans, we analyzed transmural biopsies and papillary muscles from living donors. All tissues were vibratome-sliced. By histological analysis of the transmural biopsies, we showed that tissue architecture and cellular organization were preserved. Enzymatic and vital staining methods verified viability. Optically mapped transmembrane potentials confirmed that action potential duration and morphology were similar in pig biopsies and tissue blocks. Action potential morphology and duration in human biopsies and papillary muscles agreed with published ranges. In both pigs and humans, responses to increasing pacing frequencies and ß-adrenergic stimulation were similar in transmural biopsies and larger tissues. We show that it is possible to successfully collect and characterize tissue slices from human myocardial biopsies routinely extracted from living donors, whose behavior mimics that of larger myocardial preparations both structurally and electrophysiologically.

Idioma: Inglés

DOI: 10.1038/s41598-020-77076-0

Año: 2020

Publicado en: Scientific Reports 10, 1 (2020), 19941 [13 pp]
ISSN: 2045-2322

Factor impacto JCR: 4.379 (2020)

Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 17 / 73 = 0.233 (2020) - Q1 - T1

Factor impacto SCIMAGO: 1.24 - Multidisciplinary (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/LMP124-18
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/T39-20R-BSICoS group
Financiación: info:eu-repo/grantAgreement/EUR/ERC-2014-StG-638284
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2019-105674RB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
Área (Departamento): Área Cirugía (Dpto. Cirugía)
Área (Departamento): Área Teoría Señal y Comunicac. (Dpto. Ingeniería Electrón.Com.)