000097162 001__ 97162
000097162 005__ 20210902121902.0
000097162 0247_ $$2doi$$a10.4103/aja.aja_2_20
000097162 0248_ $$2sideral$$a121136
000097162 037__ $$aART-2020-121136
000097162 041__ $$aeng
000097162 100__ $$0(orcid)0000-0003-1228-535X$$aPalacin, I.$$uUniversidad de Zaragoza
000097162 245__ $$aRelationship of sperm plasma membrane and acrosomal integrities with sperm morphometry in Bos taurus
000097162 260__ $$c2020
000097162 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097162 5203_ $$aTo date, sperm morphometric studies have assessed whole sperm populations without considering sperm function. The aim of this study was to evaluate the relationship of sperm membrane and acrosomal integrity with sperm morphometry in liquid semen samples collected from bulls. To this end, sperm morphometry was performed on cryopreserved semen samples from 16 bulls by a combination of fluorescent dyes, including Hoechst 33343, carboxyfluorescein diacetate, and propidium iodide. This allowed discrimination of different subpopulations on the basis of sperm membrane and acrosomal integrity and analysis of the morphometrics of the sperm head, nucleus, and acrosome using a specific plug-in module created on ImageJ. Acrosomal integrity was related to sperm morphometry as the heads of spermatozoa with a damaged acrosome were significantly smaller than those with a normal acrosome (P < 0.001). In the case of spermatozoa with an intact acrosome, those with a damaged plasma membrane had a larger sperm head and acrosome than spermatozoa with an intact plasma membrane (P < 0.001). No significant differences in the sperm head size were observed between sperm subpopulations without an acrosome or in the nuclear sperm morphometry of the different subpopulations. There was a positive correlation between the sperm motility values of the samples and the morphometric parameters for intact spermatozoa. These correlations were particularly strong for the morphometric parameters of the sperm acrosome. We conclude that there are clear differences in the sperm morphometry depending on the status of the sperm membrane and acrosome and this should be considered when performing this kind of analysis.
000097162 536__ $$9info:eu-repo/grantAgreement/ES/DGA-ESF/A07-17R$$9info:eu-repo/grantAgreement/EC/ETC/EFA144-16/EU/DietaPYR2$$9info:eu-repo/grantAgreement/ES/MINECO/AGL2017-85030-R
000097162 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-sa$$uhttp://creativecommons.org/licenses/by-nc-sa/3.0/es/
000097162 590__ $$a3.285$$b2020
000097162 591__ $$aUROLOGY & NEPHROLOGY$$b37 / 90 = 0.411$$c2020$$dQ2$$eT2
000097162 591__ $$aANDROLOGY$$b3 / 8 = 0.375$$c2020$$dQ2$$eT2
000097162 592__ $$a0.7$$b2020
000097162 593__ $$aUrology$$c2020$$dQ2
000097162 593__ $$aMedicine (miscellaneous)$$c2020$$dQ2
000097162 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097162 700__ $$aSantolaria, P.
000097162 700__ $$0(orcid)0000-0003-0389-2422$$aAlquezar-Baeta, C.$$uUniversidad de Zaragoza
000097162 700__ $$aSoler, C.
000097162 700__ $$aSilvestre, M.
000097162 700__ $$0(orcid)0000-0001-5316-1703$$aYaniz, J.$$uUniversidad de Zaragoza
000097162 7102_ $$12006$$2440$$aUniversidad de Zaragoza$$bDpto. Matemáticas$$cÁrea Geometría y Topología
000097162 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000097162 773__ $$g22, 6 (2020), 578-582$$pAsian J. Androl.$$tAsian journal of andrology$$x1008-682X
000097162 8564_ $$s244683$$uhttps://zaguan.unizar.es/record/97162/files/texto_completo.pdf$$yVersión publicada
000097162 8564_ $$s1697$$uhttps://zaguan.unizar.es/record/97162/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097162 909CO $$ooai:zaguan.unizar.es:97162$$particulos$$pdriver
000097162 951__ $$a2021-09-02-10:36:38
000097162 980__ $$aARTICLE