000097179 001__ 97179
000097179 005__ 20210902121914.0
000097179 0247_ $$2doi$$a10.3390/ijms21218063
000097179 0248_ $$2sideral$$a121091
000097179 037__ $$aART-2020-121091
000097179 041__ $$aeng
000097179 100__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, M.$$uUniversidad de Zaragoza
000097179 245__ $$aVasectomy and photoperiodic regimen modify the protein profile, hormonal content and antioxidant enzymes activity of ram seminal plasma
000097179 260__ $$c2020
000097179 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097179 5203_ $$aThis work aimed to determine the contribution of the testis and epididymis and the effect of the photoperiodic regimen on ram seminal plasma (SP). Semen was collected from 15 mature rams located in an equatorial (Colombian Creole and Romney Marsh, eight intact and two vasectomized) or a temperate climate (Rasa Aragonesa, three intact and two vasectomized). SP proteins were analyzed by Bradford, SDS-PAGE and difference gel electrophoresis (DIGE). Melatonin and testosterone concentrations were quantified by ELISA, and activity of glutathione peroxidase (GPx), glutathione reductase (GRD), and catalase by enzymatic assays. Vasectomy increased protein concentration and the intensity of high molecular weight bands (p < 0.001), with no differences between breeds. DIGE revealed the absence of six proteins in vasectomized rams: angiotensin-converting enzyme, lactotransferrin, phosphoglycerate kinase, sorbitol dehydrogenase, epididymal secretory glutathione peroxidase and epididymal secretory protein E1. Vasectomy also decreased melatonin concentrations in seasonal rams, and testosterone in all of them (p < 0.001), but did not affect antioxidant enzyme activity. Equatorial rams showed lower melatonin and testosterone concentration (p < 0.01) and catalase, but higher GPx activity (p < 0.05). In conclusion, vasectomy modifies the protein profile and hormonal content of ram seminal plasma, whereas the exposure to a constant photoperiod affects hormonal concentration and antioxidant enzymes activity.
000097179 536__ $$9info:eu-repo/grantAgreement/ES/CICYT-FEDER/AGL2013-43328-P$$9info:eu-repo/grantAgreement/ES/CICYT-FEDER/AGL2017-83799-R$$9info:eu-repo/grantAgreement/ES/DGA/A07-17R
000097179 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000097179 590__ $$a5.923$$b2020
000097179 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b67 / 297 = 0.226$$c2020$$dQ1$$eT1
000097179 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b49 / 178 = 0.275$$c2020$$dQ2$$eT1
000097179 592__ $$a1.455$$b2020
000097179 593__ $$aCatalysis$$c2020$$dQ1
000097179 593__ $$aComputer Science Applications$$c2020$$dQ1
000097179 593__ $$aInorganic Chemistry$$c2020$$dQ1
000097179 593__ $$aSpectroscopy$$c2020$$dQ1
000097179 593__ $$aMolecular Biology$$c2020$$dQ1
000097179 593__ $$aOrganic Chemistry$$c2020$$dQ1
000097179 593__ $$aPhysical and Theoretical Chemistry$$c2020$$dQ1
000097179 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000097179 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097179 700__ $$aFatnassi, M.
000097179 700__ $$aTorres-Ruda, F.
000097179 700__ $$aCardozo, J.A.
000097179 700__ $$aGrajales-Lombana, H.
000097179 700__ $$aHammadi, M.
000097179 700__ $$0(orcid)0000-0003-2827-3054$$aAbecia, J.A.$$uUniversidad de Zaragoza
000097179 700__ $$0(orcid)0000-0003-1306-8517$$aMuiño-Blanco, T.$$uUniversidad de Zaragoza
000097179 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe, R.$$uUniversidad de Zaragoza
000097179 700__ $$0(orcid)0000-0001-9842-6197$$aCebrián-Pérez, J.Á.$$uUniversidad de Zaragoza
000097179 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, A.$$uUniversidad de Zaragoza
000097179 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000097179 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000097179 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000097179 773__ $$g21, 21 (2020), 8063 [13 pp]$$pInt. j. mol. sci.$$tInternational Journal of Molecular Sciences$$x1661-6596
000097179 8564_ $$s630021$$uhttps://zaguan.unizar.es/record/97179/files/texto_completo.pdf$$yVersión publicada
000097179 8564_ $$s462023$$uhttps://zaguan.unizar.es/record/97179/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097179 909CO $$ooai:zaguan.unizar.es:97179$$particulos$$pdriver
000097179 951__ $$a2021-09-02-10:44:05
000097179 980__ $$aARTICLE