000097193 001__ 97193
000097193 005__ 20210902121918.0
000097193 0247_ $$2doi$$a10.3390/ani10101935
000097193 0248_ $$2sideral$$a120959
000097193 037__ $$aART-2020-120959
000097193 041__ $$aeng
000097193 100__ $$0(orcid)0000-0001-8932-4615$$aAmanzougarene, Z.
000097193 245__ $$aFitting of the in vitro gas production technique to the study of high concentrate diets
000097193 260__ $$c2020
000097193 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097193 5203_ $$aIn vitro rumen fermentation systems are often adapted to forage feeding conditions, with pH values ranging in a range close to neutrality (between 6.5 and 7.0). Several attempts using different buffers have been made to control incubation pH in order to evaluate microbial fermentation under conditions simulating high concentrate feeding, but results have not been completely successful because of rapid exhaustion of buffering capacity. Recently, a modification of bicarbonate ion concentration in the buffer of incubation solution has been proposed, which, together with using rumen inoculum from donor ruminants given high-concentrate diets, allows for mimicking such conditions in vitro. It is important to consider that the gas volume recorded is in part directly produced from microbial fermentation of substrates, but also indirectly from the buffering capacity of the medium. Thus, the contribution of each (direct and indirect) gas source to the overall production should be estimated. Another major factor affecting fermentation is the rate of passage, but closed batch systems cannot be adapted to its consideration. Therefore, a simple semicontinuous incubation system has been developed, which studies the rate and extent of fermentation by gas production at the time it allows for controlling medium pH and rate of passage by manual replacement of incubation medium by fresh saliva without including rumen inoculum. The application of this system to studies using high concentrate feeding conditions will also be reviewed here.
000097193 536__ $$9info:eu-repo/grantAgreement/ES/MINECO-DGA-FSE/AGL2013-46820
000097193 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000097193 590__ $$a2.752$$b2020
000097193 591__ $$aVETERINARY SCIENCES$$b19 / 146 = 0.13$$c2020$$dQ1$$eT1
000097193 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b13 / 63 = 0.206$$c2020$$dQ1$$eT1
000097193 592__ $$a0.583$$b2020
000097193 593__ $$aVeterinary (miscellaneous)$$c2020$$dQ1
000097193 593__ $$aAnimal Science and Zoology$$c2020$$dQ1
000097193 655_4 $$ainfo:eu-repo/semantics/review$$vinfo:eu-repo/semantics/publishedVersion
000097193 700__ $$0(orcid)0000-0002-0712-1185$$aFondevila, M.$$uUniversidad de Zaragoza
000097193 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000097193 773__ $$g10, 10 (2020), 1935 [13 pp]$$pAnimals (Basel)$$tAnimals$$x2076-2615
000097193 8564_ $$s432760$$uhttps://zaguan.unizar.es/record/97193/files/texto_completo.pdf$$yVersión publicada
000097193 8564_ $$s511269$$uhttps://zaguan.unizar.es/record/97193/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097193 909CO $$ooai:zaguan.unizar.es:97193$$particulos$$pdriver
000097193 951__ $$a2021-09-02-10:47:11
000097193 980__ $$aARTICLE