000097223 001__ 97223
000097223 005__ 20220511133123.0
000097223 0247_ $$2doi$$a10.1080/17435390.2020.1842932
000097223 0248_ $$2sideral$$a120993
000097223 037__ $$aART-2020-120993
000097223 041__ $$aeng
000097223 100__ $$aHadrup, N.
000097223 245__ $$aPulmonary toxicity of synthetic amorphous silica–effects of porosity and copper oxide doping
000097223 260__ $$c2020
000097223 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097223 5203_ $$aMaterials can be modified for improved functionality. Our aim was to test whether pulmonary toxicity of silica nanomaterials is increased by the introduction of: a) porosity; and b) surface doping with CuO; and whether c) these modifications act synergistically. Mice were exposed by intratracheal instillation and for some doses also oropharyngeal aspiration to: 1) solid silica 100 nm; 2) porous silica 100 nm; 3) porous silica 100 nm with CuO doping; 4) solid silica 300 nm; 5) porous silica 300 nm; 6) solid silica 300 nm with CuO doping; 7) porous silica 300 nm with CuO doping; 8) CuO nanoparticles 9.8 nm; or 9) carbon black Printex 90 as benchmark. Based on a pilot study, dose levels were between 0.5 and 162 µg/mouse (0.2 and 8.1 mg/kg bw). Endpoints included pulmonary inflammation (neutrophil numbers in bronchoalveolar fluid), acute phase response, histopathology, and genotoxicity assessed by the comet assay, micronucleus test, and the gamma-H2AX assay. The porous silica materials induced greater pulmonary inflammation than their solid counterparts. A similar pattern was seen for acute phase response induction and histologic changes. This could be explained by a higher specific surface area per mass unit for the most toxic particles. CuO doping further increased the acute phase response normalized according to the deposited surface area. We identified no consistent evidence of synergism between surface area and CuO doping. In conclusion, porosity and CuO doping each increased the toxicity of silica nanomaterials and there was no indication of synergy when the modifications co-occurred.
000097223 536__ $$9info:eu-repo/grantAgreement/EC/H2020/686239/EU/Performance testing, calibration and implementation of a next generation system-of-systems Risk Governance Framework for nanomaterials/caLIBRAte$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 686239-caLIBRAte
000097223 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000097223 590__ $$a5.913$$b2020
000097223 591__ $$aTOXICOLOGY$$b10 / 93 = 0.108$$c2020$$dQ1$$eT1
000097223 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b39 / 106 = 0.368$$c2020$$dQ2$$eT2
000097223 592__ $$a1.175$$b2020
000097223 593__ $$aBiomedical Engineering$$c2020$$dQ1
000097223 593__ $$aToxicology$$c2020$$dQ1
000097223 593__ $$aNanoscience and Nanotechnology$$c2020$$dQ1
000097223 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097223 700__ $$aAimonen, K.
000097223 700__ $$aIlves, M.
000097223 700__ $$aLindberg, H.
000097223 700__ $$aAtluri, R.
000097223 700__ $$aSahlgren, N.M.
000097223 700__ $$aJacobsen, N.R.
000097223 700__ $$aBarfod, K.K.
000097223 700__ $$aBerthing, T.
000097223 700__ $$aLawlor, A.
000097223 700__ $$aNorppa, H.
000097223 700__ $$aWolff, H.
000097223 700__ $$aJensen, K.A.
000097223 700__ $$aHougaard, K.S.
000097223 700__ $$aAlenius, H.
000097223 700__ $$0(orcid)0000-0003-2936-242X$$aCatalan, J.$$uUniversidad de Zaragoza
000097223 700__ $$aVogel, U.
000097223 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000097223 773__ $$g15, 1 (2020), 96-113$$pNanotoxicology$$tNanotoxicology$$x1743-5390
000097223 8564_ $$s3296138$$uhttps://zaguan.unizar.es/record/97223/files/texto_completo.pdf$$yVersión publicada
000097223 8564_ $$s240385$$uhttps://zaguan.unizar.es/record/97223/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097223 909CO $$ooai:zaguan.unizar.es:97223$$particulos$$pdriver
000097223 951__ $$a2022-05-11-13:13:15
000097223 980__ $$aARTICLE