000097336 001__ 97336
000097336 005__ 20210902121620.0
000097336 0247_ $$2doi$$a10.1111/tbed.13470
000097336 0248_ $$2sideral$$a116556
000097336 037__ $$aART-2020-116556
000097336 041__ $$aeng
000097336 100__ $$0(orcid)0000-0002-4213-2904$$aSevilla, Eloísa
000097336 245__ $$aWild griffon vultures (Gyps fulvus) fed at supplementary feeding stations: Potential carriers of pig pathogens and pig-derived antimicrobial resistance?
000097336 260__ $$c2020
000097336 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097336 5203_ $$aThe carriage of two important pathogens of pigs, that is enterotoxigenic Escherichia coli (ETEC) and Clostridioides difficile, was investigated in 104 cloacal samples from wild griffon vultures (Gyps fulvus) fed on pig carcasses at supplementary feeding stations (SFS), along with their level of antimicrobial resistance (AMR). E. coli was isolated from 90 (86.5%) samples, but no ETEC was detected, likely because ETEC fimbriae confer the species specificity of the pathogen. Resistance to at least one antimicrobial agent was detected in 89.9% of E. coli isolates, with AMR levels being extremely high (>70%) for tetracycline and streptomycin and very high (>50%) for ampicillin and sulfamethoxazole–trimethoprim. Resistance to other critically important antimicrobials such as colistin and extended-spectrum cephalosporins was 2.2% and 1.1%, respectively, and was encoded by the mcr-1 and blaSHV-12 genes. Multidrug resistance was displayed by 80% of the resistant E. coli, and blaSHV-12 gene shared plasmid with other AMR genes. In general, resistance patterns in E. coli from vultures mirrored those found in pigs. Clostridioides difficile was detected in three samples (2.9%); two of them belonged to PCR ribotype 078 and one to PCR ribotype 126, both commonly found in pigs. All C. difficile isolates were characterized by a moderate-to-high level of resistance to fluoroquinolones and macrolides but susceptible to metronidazole or vancomycin, similar to what is usually found in C. difficile isolates from pigs. Thus, vultures may contribute somewhat to the environmental dissemination of some pig pathogens through their acquisition from pig carcasses and, more importantly, of AMR for antibiotics of critical importance for humans. However, the role of vultures would likely be much lesser than that of disposing pig carcasses at the SFS. The monitoring of AMR, and particularly of colistin-resistant and ESBL-producing E. coli, should be considered in pig farms used as sources of carcasses for SFS.
000097336 536__ $$9info:eu-repo/grantAgreement/ES/MICIU/FPU14-02035
000097336 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000097336 590__ $$a5.005$$b2020
000097336 591__ $$aVETERINARY SCIENCES$$b4 / 146 = 0.027$$c2020$$dQ1$$eT1
000097336 591__ $$aINFECTIOUS DISEASES$$b23 / 92 = 0.25$$c2020$$dQ1$$eT1
000097336 592__ $$a1.391$$b2020
000097336 593__ $$aVeterinary (miscellaneous)$$c2020$$dQ1
000097336 593__ $$aMedicine (miscellaneous)$$c2020$$dQ1
000097336 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000097336 700__ $$aMarín, Clara
000097336 700__ $$aDelgado-Blas, José F.
000097336 700__ $$aGonzález-Zorn, Bruno
000097336 700__ $$aVega, Santiago
000097336 700__ $$aKuijper, Ed
000097336 700__ $$0(orcid)0000-0002-2746-3932$$aBolea, Rosa$$uUniversidad de Zaragoza
000097336 700__ $$0(orcid)0000-0001-5442-7702$$aMainar-Jaime, Raúl C.$$uUniversidad de Zaragoza
000097336 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000097336 773__ $$g67, 3 (2020), 1295-1305$$pTransboundary and Emerging Diseases$$tTransboundary and Emerging Diseases$$x1865-1674
000097336 8564_ $$s770772$$uhttps://zaguan.unizar.es/record/97336/files/texto_completo.pdf$$yPostprint
000097336 8564_ $$s253182$$uhttps://zaguan.unizar.es/record/97336/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000097336 909CO $$ooai:zaguan.unizar.es:97336$$particulos$$pdriver
000097336 951__ $$a2021-09-02-08:46:54
000097336 980__ $$aARTICLE