000097428 001__ 97428
000097428 005__ 20220617151028.0
000097428 0247_ $$2doi$$a10.3390/cancers12123767
000097428 0248_ $$2sideral$$a121693
000097428 037__ $$aART-2020-121693
000097428 041__ $$aeng
000097428 100__ $$aMedina, T.P.
000097428 245__ $$aLipid-iron nanoparticle with a cell stress release mechanism combined with a local alternating magnetic field enables site-activated drug release
000097428 260__ $$c2020
000097428 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097428 5203_ $$aMost available cancer chemotherapies are based on systemically administered small organic molecules, and only a tiny fraction of the drug reaches the disease site. The approach causes significant side effects and limits the outcome of the therapy. Targeted drug delivery provides an alternative to improve the situation. However, due to the poor release characteristics of the delivery systems, limitations remain. This report presents a new approach to address the challenges using two fundamentally different mechanisms to trigger the release from the liposomal carrier. We use an endogenous disease marker, an enzyme, combined with an externally applied magnetic field, to open the delivery system at the correct time only in the disease site. This site-activated release system is a novel two-switch nanomachine that can be regulated by a cell stress-induced enzyme at the cellular level and be remotely controlled using an applied magnetic field. We tested the concept using sphingomyelin-containing liposomes encapsulated with indocyanine green, fluorescent marker, or the anticancer drug cisplatin. We engineered the liposomes by adding paramagnetic beads to act as a receiver of outside magnetic energy. The developed multifunctional liposomes were characterized in vitro in leakage studies and cell internalization studies. The release system was further studied in vivo in imaging and therapy trials using a squamous cell carcinoma tumor in the mouse as a disease model. In vitro studies showed an increased release of loaded material when stress-related enzyme and magnetic field was applied to the carrier liposomes. The theranostic liposomes were found in tumors, and the improved therapeutic effect was shown in the survival studies.
000097428 536__ $$9info:eu-repo/grantAgreement/EUR/ERDF/122 09 053
000097428 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000097428 590__ $$a6.639$$b2020
000097428 591__ $$aONCOLOGY$$b51 / 242 = 0.211$$c2020$$dQ1$$eT1
000097428 592__ $$a1.818$$b2020
000097428 593__ $$aOncology$$c2020$$dQ1
000097428 593__ $$aCancer Research$$c2020$$dQ1
000097428 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097428 700__ $$aGerle, M.
000097428 700__ $$aHumbert, J.
000097428 700__ $$aChu, H.
000097428 700__ $$aKöpnick, A.L.
000097428 700__ $$aBarkmann, R.
000097428 700__ $$aGaramus, V.M.
000097428 700__ $$0(orcid)0000-0002-5578-7635$$aSanz, B.
000097428 700__ $$aPurcz, N.
000097428 700__ $$aWill, O.
000097428 700__ $$aAppold, L.
000097428 700__ $$aDamm, T.
000097428 700__ $$aSuojanen, J.
000097428 700__ $$aArnold, P.
000097428 700__ $$aLucius, R.
000097428 700__ $$aWillumeit-Römer, R.
000097428 700__ $$aAçil, Y.
000097428 700__ $$aWiltfang, J.
000097428 700__ $$0(orcid)0000-0003-1558-9279$$aGoya, G.F.$$uUniversidad de Zaragoza
000097428 700__ $$aGlüer, C.C.
000097428 700__ $$aMedina, O.P.
000097428 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000097428 773__ $$g12, 12 (2020), 3767 [1-27]$$pCancers$$tCancers$$x2072-6694
000097428 8564_ $$s776901$$uhttps://zaguan.unizar.es/record/97428/files/texto_completo.pdf$$yVersión publicada
000097428 8564_ $$s492899$$uhttps://zaguan.unizar.es/record/97428/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097428 909CO $$ooai:zaguan.unizar.es:97428$$particulos$$pdriver
000097428 951__ $$a2022-06-17-15:02:38
000097428 980__ $$aARTICLE