000097430 001__ 97430
000097430 005__ 20230921135437.0
000097430 0247_ $$2doi$$a10.3390/vaccines8040702
000097430 0248_ $$2sideral$$a121696
000097430 037__ $$aART-2020-121696
000097430 041__ $$aeng
000097430 100__ $$aMateos-Hernández, L.
000097430 245__ $$aAnti-tick microbiota vaccine impacts ixodes ricinus performance during feeding
000097430 260__ $$c2020
000097430 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097430 5203_ $$aThe tick microbiota is a highly complex ensemble of interacting microorganisms. Keystone taxa, with a central role in the microbial networks, support the stability and fitness of the microbial communities. The keystoneness of taxa in the tick microbiota can be inferred from microbial co-occurrence networks. Microbes with high centrality indexes are highly connected with other taxa of the microbiota and are expected to provide important resources to the microbial community and/or the tick. We reasoned that disturbance of vector microbiota by removal of ubiquitous and abundant keystone bacteria may disrupt the tick-microbiota homeostasis causing harm to the tick host. These observations and reasoning prompted us to test the hypothesis that antibodies targeting keystone bacteria may harm the ticks during feeding on immunized hosts. To this aim, in silico analyses were conducted to identify keystone bacteria in the microbiota of Ixodes nymphs. The family Enterobacteriaceae was among the top keystone taxa identified in Ixodes microbiota. Immunization of a-1, 3-galactosyltransferase-deficient-C57BL/6 (a1, 3GT KO) mice with a live vaccine containing the Enterobacteriaceae bacterium Escherichia coli strain BL21 revealed that the production of anti-E. coli and anti-a-Gal IgM and IgG was associated with high mortality of I. ricinus nymphs during feeding. However, this effect was absent in two different strains of wild type mice, BALB/c and C57BL/6. This result concurred with a wide distribution of a-1, 3-galactosyltransferase genes, and possibly a-Gal, in Enterobacteriaceae and other bacteria of tick microbiota. Interestingly, the weight of I. ricinus nymphs that fed on E. coli-immunized C57BL/6 was significantly higher than the weight of ticks that fed on C57BL/6 immunized with a mock vaccine. Our results suggest that anti-tick microbiota vaccines are a promising tool for the experimental manipulation of vector microbiota, and potentially the control of ticks and tick-borne pathogens.
000097430 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000097430 590__ $$a4.422$$b2020
000097430 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b63 / 140 = 0.45$$c2020$$dQ2$$eT2
000097430 591__ $$aIMMUNOLOGY$$b74 / 162 = 0.457$$c2020$$dQ2$$eT2
000097430 592__ $$a1.296$$b2020
000097430 593__ $$aDrug Discovery$$c2020$$dQ1
000097430 593__ $$aImmunology$$c2020$$dQ1
000097430 593__ $$aPharmacology (medical)$$c2020$$dQ1
000097430 593__ $$aPharmacology$$c2020$$dQ1
000097430 593__ $$aInfectious Diseases$$c2020$$dQ1
000097430 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097430 700__ $$aObregón, D.
000097430 700__ $$aMaye, J.
000097430 700__ $$aBorneres, J.
000097430 700__ $$aVersille, N.
000097430 700__ $$aFuente, J.L.
000097430 700__ $$0(orcid)0000-0001-7483-046X$$aEstrada-Peña, A.$$uUniversidad de Zaragoza
000097430 700__ $$aHodžic, A.
000097430 700__ $$aŠimo, L.
000097430 700__ $$aCabezas-Cruz, A.
000097430 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000097430 773__ $$g8, 4 (2020), 702 [21 pp.]$$tVaccines$$x2076-393X
000097430 8564_ $$s721263$$uhttps://zaguan.unizar.es/record/97430/files/texto_completo.pdf$$yVersión publicada
000097430 8564_ $$s491340$$uhttps://zaguan.unizar.es/record/97430/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097430 909CO $$ooai:zaguan.unizar.es:97430$$particulos$$pdriver
000097430 951__ $$a2023-09-21-13:31:08
000097430 980__ $$aARTICLE