000097462 001__ 97462
000097462 005__ 20210902121838.0
000097462 0247_ $$2doi$$a10.3390/ani10122448
000097462 0248_ $$2sideral$$a121659
000097462 037__ $$aART-2020-121659
000097462 041__ $$aeng
000097462 100__ $$aLakhssassi, K.
000097462 245__ $$aThe LEPR gene is associated with reproductive seasonality traits in Rasa Aragonesa sheep
000097462 260__ $$c2020
000097462 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097462 5203_ $$aThe aim of this study was to characterize and identify causative polymorphisms in the leptin receptor (LEPR) gene responsible for the seasonal variation of reproductive traits in sheep. Three reproductive seasonality traits were studied: the total days of anoestrous (TDA), the progesterone cycling months (P4CM) and the oestrous cycling months (OCM). In total, 18 SNPs were detected in 33 ewes with extreme values for TDA and OCM. Six SNPs were non-synonymous substitutions and two of them were predicted in silico as deleterious: rs596133197 and rs403578195. These polymorphisms were then validated in 239 ewes. The SNP rs403578195, located in exon 8 and leading to a change of alanine to glycine (Ala284Gly) in the extracellular domain of the protein, was associated with the OCM trait, being the G allele associated with a decrease of 12 percent of the OCM trait. Haplotype analyses also suggested the involvement of other non-synonymous SNP located in exon 20 (rs405459906). This SNP also produces an amino acid change (Lys1069Glu) in the intracellular domain of the protein and segregates independently of rs403578195. These results confirm for the first time the role of the LEPR gene in sheep reproductive seasonality.
000097462 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/A14-17R-GRUPO SAGAS$$9info:eu-repo/grantAgreement/ES/MCIU/BES-2017-080154$$9info:eu-repo/grantAgreement/ES/MCIU/INIA-RTA2015-00090
000097462 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000097462 590__ $$a2.752$$b2020
000097462 591__ $$aVETERINARY SCIENCES$$b19 / 146 = 0.13$$c2020$$dQ1$$eT1
000097462 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b13 / 63 = 0.206$$c2020$$dQ1$$eT1
000097462 592__ $$a0.583$$b2020
000097462 593__ $$aVeterinary (miscellaneous)$$c2020$$dQ1
000097462 593__ $$aAnimal Science and Zoology$$c2020$$dQ1
000097462 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097462 700__ $$aSerrano, M.
000097462 700__ $$aLahoz, B.
000097462 700__ $$aSarto, M.P.
000097462 700__ $$0(orcid)0000-0001-6042-3134$$aIguácel, L.P.
000097462 700__ $$aFolch, J.
000097462 700__ $$0(orcid)0000-0001-5015-8103$$aAlabart, J.L.
000097462 700__ $$aCalvo, J.H.
000097462 773__ $$g10, 12 (2020), 1-15$$pAnimals (Basel)$$tAnimals$$x2076-2615
000097462 8564_ $$s450783$$uhttps://zaguan.unizar.es/record/97462/files/texto_completo.pdf$$yVersión publicada
000097462 8564_ $$s483346$$uhttps://zaguan.unizar.es/record/97462/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097462 909CO $$ooai:zaguan.unizar.es:97462$$particulos$$pdriver
000097462 951__ $$a2021-09-02-10:21:36
000097462 980__ $$aARTICLE