000097477 001__ 97477
000097477 005__ 20210902121845.0
000097477 0247_ $$2doi$$a10.1080/21505594.2020.1851940
000097477 0248_ $$2sideral$$a121678
000097477 037__ $$aART-2020-121678
000097477 041__ $$aeng
000097477 100__ $$0(orcid)0000-0002-8134-0693$$aArenas, J.$$uUniversidad de Zaragoza
000097477 245__ $$aThe outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB
000097477 260__ $$c2020
000097477 5060_ $$aAccess copy available to the general public$$fUnrestricted
000097477 5203_ $$aMafB proteins are toxins secreted by Neisseria spp. which are involved in interbacterial competition. Their secretion mechanism has so far not been elucidated. Each strain can produce several MafB variants. On the chromosome, the mafB genes are localized on genomic islands also containing mafA genes. MafA proteins have a role in virulence with reported activities in adhesion and transcytosis of pathogenic Neisseria, a priori unrelated to MafB activities. In this study, we investigated the possible involvement of MafA in the transport of MafB across the outer membrane of Neisseria meningitidis. In wild-type strains, proteolytic fragments of MafB proteins were detected in the extracellular medium. In the absence of MafA, secretion was abrogated, and, in the case of MafBI, full-length and truncated polypeptides were detected inside the cells and inside outer-membrane vesicles. MafBI secretion required its cognate MafA, whereas MafBIII could use any MafA. Heterologous expression in Escherichia coli showed that MafBIII is transported to a cell-surface-exposed, i.e. protease-accessible, location in a MafA-dependent way. MafA itself was found to be localized to the outer membrane, forming large oligomeric complexes. As homologs were found in diverse bacteria, the Maf system represents a new protein secretion system in Gram-negative bacteria.
000097477 536__ $$9info:eu-repo/grantAgreement/ES/ISCIII-EDRF/PT17-0019
000097477 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000097477 590__ $$a5.882$$b2020
000097477 591__ $$aINFECTIOUS DISEASES$$b12 / 92 = 0.13$$c2020$$dQ1$$eT1
000097477 591__ $$aMICROBIOLOGY$$b23 / 136 = 0.169$$c2020$$dQ1$$eT1
000097477 591__ $$aIMMUNOLOGY$$b44 / 162 = 0.272$$c2020$$dQ2$$eT1
000097477 592__ $$a1.927$$b2020
000097477 593__ $$aImmunology$$c2020$$dQ1
000097477 593__ $$aInfectious Diseases$$c2020$$dQ1
000097477 593__ $$aParasitology$$c2020$$dQ1
000097477 593__ $$aMicrobiology (medical)$$c2020$$dQ1
000097477 593__ $$aMicrobiology$$c2020$$dQ1
000097477 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000097477 700__ $$aCatón, L.
000097477 700__ $$avan den Hoeven, T.
000097477 700__ $$ade Maat, V.
000097477 700__ $$aCruz Herrero, J.
000097477 700__ $$aTommassen, J.
000097477 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000097477 773__ $$g11, 1 (2020), 1701-1715$$pVIRULENCE$$tVirulence$$x2150-5594
000097477 8564_ $$s798581$$uhttps://zaguan.unizar.es/record/97477/files/texto_completo.pdf$$yVersión publicada
000097477 8564_ $$s175991$$uhttps://zaguan.unizar.es/record/97477/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000097477 909CO $$ooai:zaguan.unizar.es:97477$$particulos$$pdriver
000097477 951__ $$a2021-09-02-10:26:51
000097477 980__ $$aARTICLE