000098237 001__ 98237
000098237 005__ 20210121150813.0
000098237 0247_ $$2doi$$a10.3390/molecules24132422
000098237 0248_ $$2sideral$$a121883
000098237 037__ $$aART-2019-121883
000098237 041__ $$aeng
000098237 100__ $$aMiranda-Apodaca, J.
000098237 245__ $$aEmissive enhancement of the singlet oxygen chemiluminescence probe after binding to bovine serum albumin
000098237 260__ $$c2019
000098237 5060_ $$aAccess copy available to the general public$$fUnrestricted
000098237 5203_ $$aA chemiluminescence probe for singlet oxygen 1O2 (SOCL) was investigated in phosphate buffer saline (PBS), either in the absence of proteins or containing bovine serum albumin (BSA). In the protein-free PBS, the reactivity of SOCL for methylene blue (MB)-photosensitized 1O2 was found to be moderate or low. The reaction yield increased with temperature and/or concentration of dissolved molecular oxygen. Unexpectedly, the presence of BSA boosted both the emissive nature and the thermal stability of the phenoxy-dioxetane intermediate formed in the chemiexcitation pathway. Isothermal titration calorimetry showed that SOCL has a moderate binding affinity for BSA and that entropy forces drive the formation of the SOCL-BSA complex. A model with two identical and independent binding sites was used to fit the binding isotherm data. Co-operative binding was observed when MB was present. Local viscosity factors and/or conformational restrictions of the BSA-bound SOCL phenoxy-dioxetane were proposed to contribute to the formation of the highly emissive benzoate ester during the chemically initiated electron exchange luminescence (CIEEL) process. These results led us to conclude that hydrophobic interactions of the SOCL with proteins can modify the emissive nature of its phenoxy-dioxetane, which should be taken into account when using SOCL or its cell-penetrating peptide derivative in living cells.
000098237 536__ $$9info:eu-repo/grantAgreement/ES/MICINN/AGL2016-79589-R$$9info:eu-repo/grantAgreement/ES/MICINN/BFU2007-68107-C02-02/BMC$$9info:eu-repo/grantAgreement/ES/MICINN/RTC-2017-6756-2
000098237 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000098237 590__ $$a3.267$$b2019
000098237 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b70 / 177 = 0.395$$c2019$$dQ2$$eT2
000098237 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b141 / 297 = 0.475$$c2019$$dQ2$$eT2
000098237 592__ $$a0.698$$b2019
000098237 593__ $$aPharmaceutical Science$$c2019$$dQ1
000098237 593__ $$aChemistry (miscellaneous)$$c2019$$dQ2
000098237 593__ $$aDrug Discovery$$c2019$$dQ2
000098237 593__ $$aPhysical and Theoretical Chemistry$$c2019$$dQ2
000098237 593__ $$aOrganic Chemistry$$c2019$$dQ2
000098237 593__ $$aAnalytical Chemistry$$c2019$$dQ2
000098237 593__ $$aMedicine (miscellaneous)$$c2019$$dQ2
000098237 593__ $$aMolecular Medicine$$c2019$$dQ3
000098237 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000098237 700__ $$aHananya, N.
000098237 700__ $$0(orcid)0000-0001-5702-4538$$aVelázquez-Campoy, A.$$uUniversidad de Zaragoza
000098237 700__ $$aShabat, D.
000098237 700__ $$aArellano, J.B.
000098237 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000098237 773__ $$g24, 13 (2019), 2422 [16 pp]$$pMolecules$$tMolecules$$x1420-3049
000098237 8564_ $$s881329$$uhttps://zaguan.unizar.es/record/98237/files/texto_completo.pdf$$yVersión publicada
000098237 8564_ $$s2284192$$uhttps://zaguan.unizar.es/record/98237/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000098237 909CO $$ooai:zaguan.unizar.es:98237$$particulos$$pdriver
000098237 951__ $$a2021-01-21-12:24:00
000098237 980__ $$aARTICLE