000098238 001__ 98238 000098238 005__ 20230126102842.0 000098238 0247_ $$2doi$$a10.3390/foods8100489 000098238 0248_ $$2sideral$$a121884 000098238 037__ $$aART-2019-121884 000098238 041__ $$aeng 000098238 100__ $$aAntunes-Rohling, A. 000098238 245__ $$aCharacterization of the spoilage microbiota of hake fillets packaged under a modified atmosphere (MAP) rich in CO2 (50% CO2/50% N2) and stored at different temperatures 000098238 260__ $$c2019 000098238 5060_ $$aAccess copy available to the general public$$fUnrestricted 000098238 5203_ $$aThe aim of this study was to characterize the spoilage microbiota of hake fillets stored under modified atmospheres (MAP) (50% CO2/50% N2) at different temperatures using high-throughput 16S rRNA gene sequencing and to compare the results with those obtained using traditional microbiology techniques. The results obtained indicate that, as expected, higher storage temperatures lead to shorter shelf-lives (the time of sensory rejection by panelists). Thus, the shelf-life decreased from six days to two days for Batch A when the storage temperature increased from 1 to 7 °C, and from five to two days—when the same increase in storage temperature was compared—for Batch B. In all cases, the trimethylamine (TMA) levels measured at the time of sensory rejection of hake fillets exceeded the recommended threshold of 5 mg/100 g. Photobacterium and Psychrobacter were the most abundant genera at the time of spoilage in all but one of the samples analyzed: Thus, Photobacterium represented between 19% and 46%, and Psychrobacter between 27% and 38% of the total microbiota. They were followed by Moritella, Carnobacterium, Shewanella, and Vibrio, whose relative order varied depending on the sample/batch analyzed. These results highlight the relevance of Photobacterium as a spoiler of hake stored in atmospheres rich in CO2. Further research will be required to elucidate if other microorganisms, such as Psychrobacter, Moritella, or Carnobacterium, also contribute to spoilage of hake when stored under MAP. 000098238 536__ $$9info:eu-repo/grantAgreement/EC/H2020/767839/EU/ECO-INNOVATIVE PROCESSING TECHNOLOGY FOR BETTER QUALITY AND SHELF LIFE OF FISH PRODUCTS/ULTRAFISH$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 767839-ULTRAFISH 000098238 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000098238 590__ $$a4.092$$b2019 000098238 591__ $$aFOOD SCIENCE & TECHNOLOGY$$b27 / 139 = 0.194$$c2019$$dQ1$$eT1 000098238 592__ $$a0.661$$b2019 000098238 593__ $$aFood Science$$c2019$$dQ2 000098238 593__ $$aHealth (social science)$$c2019$$dQ2 000098238 593__ $$aPlant Science$$c2019$$dQ2 000098238 593__ $$aHealth Professions (miscellaneous)$$c2019$$dQ2 000098238 593__ $$aMicrobiology$$c2019$$dQ3 000098238 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000098238 700__ $$aCalero, S. 000098238 700__ $$0(orcid)0000-0002-4121-8087$$aHalaihel, N.$$uUniversidad de Zaragoza 000098238 700__ $$0(orcid)0000-0002-1905-5883$$aMarquina, P. 000098238 700__ $$0(orcid)0000-0003-3957-9091$$aRaso, J.$$uUniversidad de Zaragoza 000098238 700__ $$0(orcid)0000-0001-9660-8579$$aCalanche, J.$$uUniversidad de Zaragoza 000098238 700__ $$0(orcid)0000-0002-3764-0189$$aBeltrán, J.A.$$uUniversidad de Zaragoza 000098238 700__ $$0(orcid)0000-0003-2430-858X$$aÁlvarez, I.$$uUniversidad de Zaragoza 000098238 700__ $$0(orcid)0000-0002-5049-3646$$aCebrián, G.$$uUniversidad de Zaragoza 000098238 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal 000098238 7102_ $$12008$$2780$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Tecnología de Alimentos 000098238 773__ $$g8, 10 (2019), 489 [14 pp]$$pFoods$$tFoods$$x2304-8158 000098238 8564_ $$s581419$$uhttps://zaguan.unizar.es/record/98238/files/texto_completo.pdf$$yVersión publicada 000098238 8564_ $$s2428283$$uhttps://zaguan.unizar.es/record/98238/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000098238 909CO $$ooai:zaguan.unizar.es:98238$$particulos$$pdriver 000098238 951__ $$a2023-01-26-09:57:48 000098238 980__ $$aARTICLE