000098313 001__ 98313 000098313 005__ 20240118091947.0 000098313 0248_ $$2sideral$$a122055 000098313 037__ $$aART-2019-122055 000098313 041__ $$aeng 000098313 100__ $$0(orcid)0000-0002-1481-9805$$aCequier, A.$$uUniversidad de Zaragoza 000098313 245__ $$aHumoral immune response against allogeneic equine mesenchymal stem cells (MSCs) mediated by the major histocompatibility complex (MHC): an issue to take into account for the safety and efficacy of treatment with MSCs 000098313 260__ $$c2019 000098313 5060_ $$aAccess copy available to the general public$$fUnrestricted 000098313 5203_ $$aAllogeneic mesenchymal stem cells (MSCs) present several advantages, but recipient immune response needs to be further elucidated. Proinflammatory priming of MSCs activated their in vivo regulatory capacity, but repeated administrations led to slight inflammatory reaction in an osteoarthritis equine model. This may be associated with higher major histocompatibility complex (MHC) expression, which would increase MSC immunogenicity potentially inducing humoral mediated immune memory. This study aimed at assessing allo-antibody production against donor’s equine MHC (equine leukocyte antigen, ELA) in animals that received intra-articular repeated administration of allogeneic MSC-primed. For this purpose, we used stored samples from a previous study. Donor and recipients ELA-haplotypes were stablished by microsatellite typing and complementmediated microcytoxicity assays were carried out by exposing target cells from the donor (unstimulated MSCs [MSC-nai¨ve], MSC-primed or lymphocytes [control]) to sera collected at different time-points from 10 recipients: ELA-mismatched MSCnai ¨ve recipients, ELA-mismatched MSC-primed recipients or ELA-partially matched MSC-primed recipients. All animals receiving allogeneic MSCs produced allo-antibodies after the first injection, regardless of the matching degree. However, antibody peak production after second administration was only observed in ELA-mismatched recipients, both of MSC-nai¨ve and MSCprimed. Horses injected with MSC-primed produced fewer antibodies but MSC-primed were more targeted in the microcytoxicity assay. Thus, activated immunomodulatory profile of MSC-primed could have led to slighter humoral response after ... 000098313 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000098313 590__ $$a4.273$$b2019 000098313 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b30 / 156 = 0.192$$c2019$$dQ1$$eT1 000098313 591__ $$aGENETICS & HEREDITY$$b38 / 176 = 0.216$$c2019$$dQ1$$eT1 000098313 591__ $$aMEDICINE, RESEARCH & EXPERIMENTAL$$b40 / 138 = 0.29$$c2019$$dQ2$$eT1 000098313 592__ $$a1.648$$b2019 000098313 593__ $$aGenetics$$c2019$$dQ1 000098313 593__ $$aMolecular Medicine$$c2019$$dQ1 000098313 593__ $$aMolecular Biology$$c2019$$dQ2 000098313 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000098313 700__ $$0(orcid)0000-0001-9818-508X$$aBarrachina, L.$$uUniversidad de Zaragoza 000098313 700__ $$0(orcid)0000-0001-7188-0461$$aRomero, A.$$uUniversidad de Zaragoza 000098313 700__ $$0(orcid)0000-0003-1286-4968$$aVitoria, A.$$uUniversidad de Zaragoza 000098313 700__ $$0(orcid)0000-0001-5740-0185$$aZaragoza, P.$$uUniversidad de Zaragoza 000098313 700__ $$0(orcid)0000-0002-8712-2275$$aVazquez, F.J.$$uUniversidad de Zaragoza 000098313 700__ $$0(orcid)0000-0003-3289-2675$$aRodellar, C.$$uUniversidad de Zaragoza 000098313 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética 000098313 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal 000098313 773__ $$g30, 11 (2019), A164$$pHum. gene ther.$$tHUMAN GENE THERAPY$$x1043-0342 000098313 85641 $$uhttps://www.liebertpub.com/doi/pdf/10.1089/hum.2019.29095.abstracts$$zTexto completo de la revista 000098313 8564_ $$s59723$$uhttps://zaguan.unizar.es/record/98313/files/texto_completo.pdf$$yVersión publicada 000098313 8564_ $$s3449429$$uhttps://zaguan.unizar.es/record/98313/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000098313 909CO $$ooai:zaguan.unizar.es:98313$$particulos$$pdriver 000098313 951__ $$a2024-01-18-09:11:49 000098313 980__ $$aARTICLE