Combination of exosomes and near-infrared responsive gold nanoparticles: new selective and specific therapeutic vehicle
Sancho-Albero, M. (Universidad de Zaragoza) ; Navascues, N. ; Mendoza, G. ; Sebastian, V. (Universidad de Zaragoza) ; Arruebo, M. (Universidad de Zaragoza) ; Martin-Duque, P. ; Santamaria, J. (Universidad de Zaragoza)
Resumen: P602
Exosomes are extracellular vesicles (50 -150 nm of diameter) considered key elements for the intercellular communication. Although they are proposed to be ideal vehicles for the targeting of novel therapies, very little is known about the selectiveness and specificity of the transference processes involving exosomes released from different cells. PEGylated Hollow gold nanoparticles (PEG-HGNs) are near-infrared (NIR) responsive nanoparticles (NPs) which are able to generate localized heat by the use of NIR light leading to cell death when applying optical hyperthermia. In this study, we demonstrate the selectivity of in vitro exosomal transfer between certain cell types and how this phenomenon can be exploited to develop new specific vectors for advanced therapies. Firstly, PEG-HGNs were successfully incorporated in the exosome biogenesis pathway of placental stem cells (MSCs) and they were released as PEG-HGNs-loaded exosomes (PEGHGNs_ MSCs_EXOs). Exosomes were characterized by confocal microscopy, western blot, nanosight, zeta potential and electronic microscopy. Afterwards, time lapse microscopy and atomic emission spectroscopy demonstrated the selective transfer of the ...
Idioma: Inglés
Año: 2019
Publicado en: HUMAN GENE THERAPY 30, 11 (2019), A192
ISSN: 1043-0342
Originalmente disponible en: Texto completo de la revista
Factor impacto JCR: 4.273 (2019)
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 30 / 156 = 0.192 (2019) - Q1 - T1
Categ. JCR: GENETICS & HEREDITY rank: 38 / 177 = 0.215 (2019) - Q1 - T1
Categ. JCR: MEDICINE, RESEARCH & EXPERIMENTAL rank: 40 / 138 = 0.29 (2019) - Q2 - T1
Factor impacto SCIMAGO: 1.648 - Genetics (Q1) - Molecular Medicine (Q1) - Molecular Biology (Q2)
Tipo y forma: Comunicación congreso (Versión definitiva)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
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Exosomes are extracellular vesicles (50 -150 nm of diameter) considered key elements for the intercellular communication. Although they are proposed to be ideal vehicles for the targeting of novel therapies, very little is known about the selectiveness and specificity of the transference processes involving exosomes released from different cells. PEGylated Hollow gold nanoparticles (PEG-HGNs) are near-infrared (NIR) responsive nanoparticles (NPs) which are able to generate localized heat by the use of NIR light leading to cell death when applying optical hyperthermia. In this study, we demonstrate the selectivity of in vitro exosomal transfer between certain cell types and how this phenomenon can be exploited to develop new specific vectors for advanced therapies. Firstly, PEG-HGNs were successfully incorporated in the exosome biogenesis pathway of placental stem cells (MSCs) and they were released as PEG-HGNs-loaded exosomes (PEGHGNs_ MSCs_EXOs). Exosomes were characterized by confocal microscopy, western blot, nanosight, zeta potential and electronic microscopy. Afterwards, time lapse microscopy and atomic emission spectroscopy demonstrated the selective transfer of the ...
Idioma: Inglés
Año: 2019
Publicado en: HUMAN GENE THERAPY 30, 11 (2019), A192
ISSN: 1043-0342
Originalmente disponible en: Texto completo de la revista
Factor impacto JCR: 4.273 (2019)
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 30 / 156 = 0.192 (2019) - Q1 - T1
Categ. JCR: GENETICS & HEREDITY rank: 38 / 177 = 0.215 (2019) - Q1 - T1
Categ. JCR: MEDICINE, RESEARCH & EXPERIMENTAL rank: 40 / 138 = 0.29 (2019) - Q2 - T1
Factor impacto SCIMAGO: 1.648 - Genetics (Q1) - Molecular Medicine (Q1) - Molecular Biology (Q2)
Tipo y forma: Comunicación congreso (Versión definitiva)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.Exportado de SIDERAL (2021-01-26-15:09:18)
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Registro creado el 2021-01-26, última modificación el 2021-01-26