000098457 001__ 98457
000098457 005__ 20240122154814.0
000098457 0247_ $$2doi$$a10.1152/ajpendo.00199.2019
000098457 0248_ $$2sideral$$a116094
000098457 037__ $$aART-2020-116094
000098457 041__ $$aeng
000098457 100__ $$0(orcid)0000-0002-4665-9674$$aHerrera-Marcos, Luis V.
000098457 245__ $$aPgc1a is responsible for the sex differences in hepatic Cidec/Fsp27ß mRNA expression in hepatic steatosis of mice fed a western diet
000098457 260__ $$c2020
000098457 5060_ $$aAccess copy available to the general public$$fUnrestricted
000098457 5203_ $$aHepatic fat-specific protein 27 (Cidec/Fsp27) mRNA levels have been associated with hepatic lipid droplet extent under certain circumstances. To address its hepatic expression under different dietary conditions and in both sexes, Apoe-deficient mice were subjected to different experimental conditions for 11 weeks to test the influence of cholesterol, Western diet, squalene, oleanolic acid, sex and surgical castration on Cidec/Fsp27 mRNA expression. Dietary cholesterol increased hepatic Cidec/Fsp27ß expression, an effect that was suppressed when cholesterol was combined with saturated fat as represented by Western-diet feeding. Using the latter diet, oleanolic acid or squalene did not modify its expression. Females showed lower levels of hepatic Cidec/Fsp27ß expression than males when they were fed Western diets, a result that was translated into lesser amount of CIDEC/FSP27 protein in lipid droplets and microsomes. This was also confirmed in Ldlr-deficient mice. Incubation with estradiol resulted in decreased Cidec/Fsp27ß expression in AML12 cells. While male surgical castration did not modify the expression, ovariectomized females did show increased levels compared to control females. Females also showed increased expression of Pgc1a, suppressed by ovariectomy, and the values were significantly and inversely associated with those of Cidec/Fsp27ß. When Pgc1a-deficient mice were used, the sex differences on Cidec/Fsp27ß expression disappeared. Therefore, hepatic Cidec/Fsp27ß expression has a complex regulation influenced by diet and sex hormonal milieu. The mRNA sex differences are controlled by Pgc1a.
000098457 536__ $$9info:eu-repo/grantAgreement/ES/DGA/B16-R17$$9info:eu-repo/grantAgreement/EC/H2020/115916/EU/Training European Network: Metabolic Dysfunctions associated with Pharmacological Treatment of Schizophrenia/TREATMENT$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 721236-TREATMENT$$9info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012$$9info:eu-repo/grantAgreement/ES/MICINN/RTI2018-093864-B-100$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2015-63904-R$$9info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R
000098457 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000098457 590__ $$a4.31$$b2020
000098457 591__ $$aPHYSIOLOGY$$b15 / 81 = 0.185$$c2020$$dQ1$$eT1
000098457 591__ $$aENDOCRINOLOGY & METABOLISM$$b57 / 144 = 0.396$$c2020$$dQ2$$eT2
000098457 592__ $$a1.506$$b2020
000098457 593__ $$aEndocrinology, Diabetes and Metabolism$$c2020$$dQ1
000098457 593__ $$aPhysiology (medical)$$c2020$$dQ1
000098457 593__ $$aPhysiology$$c2020$$dQ1
000098457 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000098457 700__ $$aSancho-Knapik, Sara
000098457 700__ $$0(orcid)0000-0002-2348-8702$$aGabás-Rivera, Clara
000098457 700__ $$0(orcid)0000-0002-8442-8041$$aBarranquero, Cristina
000098457 700__ $$aGascón, Sonia$$uUniversidad de Zaragoza
000098457 700__ $$aRomanos, Eduardo$$uUniversidad de Zaragoza
000098457 700__ $$0(orcid)0000-0002-8100-5596$$aMartínez-Beamonte, Roberto$$uUniversidad de Zaragoza
000098457 700__ $$0(orcid)0000-0002-0108-1004$$aNavarro, María A.$$uUniversidad de Zaragoza
000098457 700__ $$0(orcid)0000-0002-5841-0462$$aSurra, Joaquín C.$$uUniversidad de Zaragoza
000098457 700__ $$0(orcid)0000-0003-1197-4276$$aArnal, Carmen$$uUniversidad de Zaragoza
000098457 700__ $$0(orcid)0000-0002-4156-9012$$aGarcía-de-Jalón, José A.$$uUniversidad de Zaragoza
000098457 700__ $$0(orcid)0000-0002-3595-7668$$aRodríguez-Yoldi, María J.$$uUniversidad de Zaragoza
000098457 700__ $$aTena-Sempere, Manuel
000098457 700__ $$aSánchez-Ramos, Cristina
000098457 700__ $$aMonsalve, María
000098457 700__ $$0(orcid)0000-0002-8251-8457$$aOsada, Jesús$$uUniversidad de Zaragoza
000098457 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000098457 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000098457 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000098457 7102_ $$11012$$2410$$aUniversidad de Zaragoza$$bDpto. Farmac.Fisiol.y Med.L.F.$$cÁrea Fisiología
000098457 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000098457 7102_ $$11009$$2X$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Técnica. Lab. y Talleres
000098457 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000098457 773__ $$g318, 2 (2020), E249–E261$$pAm. j. physiol: endocrinol. metab.$$tAMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM$$x0193-1849
000098457 8564_ $$s2995273$$uhttps://zaguan.unizar.es/record/98457/files/texto_completo.pdf$$yPostprint
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000098457 909CO $$ooai:zaguan.unizar.es:98457$$particulos$$pdriver
000098457 951__ $$a2024-01-22-15:33:08
000098457 980__ $$aARTICLE