98457 20240122154814.0 doi 10.1152/ajpendo.00199.2019 sideral 116094 ART-2020-116094 eng Herrera-Marcos, Luis V. (orcid)0000-0002-4665-9674 Pgc1a is responsible for the sex differences in hepatic Cidec/Fsp27ß mRNA expression in hepatic steatosis of mice fed a western diet 2020 Access copy available to the general public Unrestricted Hepatic fat-specific protein 27 (Cidec/Fsp27) mRNA levels have been associated with hepatic lipid droplet extent under certain circumstances. To address its hepatic expression under different dietary conditions and in both sexes, Apoe-deficient mice were subjected to different experimental conditions for 11 weeks to test the influence of cholesterol, Western diet, squalene, oleanolic acid, sex and surgical castration on Cidec/Fsp27 mRNA expression. Dietary cholesterol increased hepatic Cidec/Fsp27ß expression, an effect that was suppressed when cholesterol was combined with saturated fat as represented by Western-diet feeding. Using the latter diet, oleanolic acid or squalene did not modify its expression. Females showed lower levels of hepatic Cidec/Fsp27ß expression than males when they were fed Western diets, a result that was translated into lesser amount of CIDEC/FSP27 protein in lipid droplets and microsomes. This was also confirmed in Ldlr-deficient mice. Incubation with estradiol resulted in decreased Cidec/Fsp27ß expression in AML12 cells. While male surgical castration did not modify the expression, ovariectomized females did show increased levels compared to control females. Females also showed increased expression of Pgc1a, suppressed by ovariectomy, and the values were significantly and inversely associated with those of Cidec/Fsp27ß. When Pgc1a-deficient mice were used, the sex differences on Cidec/Fsp27ß expression disappeared. Therefore, hepatic Cidec/Fsp27ß expression has a complex regulation influenced by diet and sex hormonal milieu. The mRNA sex differences are controlled by Pgc1a. info:eu-repo/grantAgreement/ES/DGA/B16-R17 info:eu-repo/grantAgreement/EC/H2020/115916/EU/Training European Network: Metabolic Dysfunctions associated with Pharmacological Treatment of Schizophrenia/TREATMENT This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 721236-TREATMENT info:eu-repo/grantAgreement/ES/ISCIII/CB06-03-1012 info:eu-repo/grantAgreement/ES/MICINN/RTI2018-093864-B-100 info:eu-repo/grantAgreement/ES/MINECO/SAF2015-63904-R info:eu-repo/grantAgreement/ES/MINECO/SAF2016-75441-R info:eu-repo/semantics/openAccess All rights reserved http://www.europeana.eu/rights/rr-f/ 4.31 2020 PHYSIOLOGY 15 / 81 = 0.185 2020 Q1 T1 ENDOCRINOLOGY & METABOLISM 57 / 144 = 0.396 2020 Q2 T2 1.506 2020 Endocrinology, Diabetes and Metabolism 2020 Q1 Physiology (medical) 2020 Q1 Physiology 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Sancho-Knapik, Sara Gabás-Rivera, Clara (orcid)0000-0002-2348-8702 Barranquero, Cristina (orcid)0000-0002-8442-8041 Gascón, Sonia Universidad de Zaragoza Romanos, Eduardo Universidad de Zaragoza Martínez-Beamonte, Roberto Universidad de Zaragoza (orcid)0000-0002-8100-5596 Navarro, María A. Universidad de Zaragoza (orcid)0000-0002-0108-1004 Surra, Joaquín C. Universidad de Zaragoza (orcid)0000-0002-5841-0462 Arnal, Carmen Universidad de Zaragoza (orcid)0000-0003-1197-4276 García-de-Jalón, José A. Universidad de Zaragoza (orcid)0000-0002-4156-9012 Rodríguez-Yoldi, María J. Universidad de Zaragoza (orcid)0000-0002-3595-7668 Tena-Sempere, Manuel Sánchez-Ramos, Cristina Monsalve, María Osada, Jesús Universidad de Zaragoza (orcid)0000-0002-8251-8457 1001 420 Universidad de Zaragoza Dpto. Anatom.,Embri.Genét.Ani. Área Genética 2008 640 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Nutrición Bromatología 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1012 410 Universidad de Zaragoza Dpto. Farmac.Fisiol.y Med.L.F. Área Fisiología 2008 700 Universidad de Zaragoza Dpto. Produc.Animal Cienc.Ali. Área Producción Animal 1009 X Universidad de Zaragoza Dpto. Patología Animal Área Técnica. Lab. y Talleres 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 318, 2 (2020), E249–E261 Am. j. physiol: endocrinol. metab. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 0193-1849 2995273 http://zaguan.unizar.es/record/98457/files/texto_completo.pdf Postprint 1373794 http://zaguan.unizar.es/record/98457/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:98457 articulos driver 2024-01-22-15:33:08 ARTICLE