99058 20230519145437.0 doi 10.3390/jpm11010025 sideral 122309 ART-2021-122309 eng Hermoso-Durán, S. Thermal liquid biopsy (TLB) focused on benign and premalignant pancreatic cyst diagnosis 2021 Access copy available to the general public Unrestricted Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this type of pathology. Methods: In this work, twenty patients (12 males and 8 females, average ages 62) diagnosed with a pancreatic cyst benign (10) and premalignant (10) cyst lesions were recruited, and biological samples were obtained during the endoscopic ultrasonography procedure. Results: Proteomic content of cyst liquid samples was studied and several common proteins in the different groups were identified. TLB cyst liquid profiles reflected protein content. Also, TLB serum score was able to discriminate between healthy and cysts patients (71% sensitivity and 98% specificity) and between benign and premalignant cysts (75% sensitivity and 67% specificity). Conclusions: TLB analysis of plasmatic serum sample, a quick, simple and non-invasive technique that can be easily implemented, reports valuable information on the observed pancreatic lesion. These preliminary results set the basis for a larger study to refine TLB serum score and move closer to the clinical application of TLB providing useful information to the gastroenterologist during patient diagnosis. info:eu-repo/grantAgreement/ES/DGA/E45-17R info:eu-repo/grantAgreement/ES/FIS/PI15-00663 info:eu-repo/grantAgreement/ES/ISCIII/CIBERehd info:eu-repo/grantAgreement/ES/ISCIII/CPII13-00017 info:eu-repo/grantAgreement/ES/ISCIII-ESF/Investing in your future info:eu-repo/grantAgreement/ES/ISCIII-FIS/FI19-00146 info:eu-repo/grantAgreement/ES/ISCIII-FIS/PI18-00349 info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BFU2016-78232-P info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 3.508 2021 MEDICINE, GENERAL & INTERNAL 69 / 172 = 0.401 2021 Q2 T2 HEALTH CARE SCIENCES & SERVICES 42 / 109 = 0.385 2021 Q2 T2 0.757 2021 Medicine (miscellaneous) 2021 Q2 1.8 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion García-Rayado, G. Ceballos-Laita, L. Sostres, C. Universidad de Zaragoza (orcid)0000-0001-7466-3876 Vega, S. (orcid)0000-0002-1232-6310 Millastre, J. Sánchez-Gracia, O. Ojeda, J.L. Universidad de Zaragoza (orcid)0000-0003-0853-2880 Lanas, Á. Universidad de Zaragoza (orcid)0000-0001-5932-2889 Velázquez-Campoy, A. Universidad de Zaragoza (orcid)0000-0001-5702-4538 Abian, O. Universidad de Zaragoza (orcid)0000-0001-5664-1729 2007 265 Universidad de Zaragoza Dpto. Métodos Estadísticos Área Estadís. Investig. Opera. 1007 610 Universidad de Zaragoza Dpto. Medicina, Psiqu. y Derm. Area Medicina 1002 060 Universidad de Zaragoza Dpto. Bioq.Biolog.Mol. Celular Área Bioquímica y Biolog.Mole. 11, 1 (2021), 25 [19 pp] J. pers. med. Journal of Personalized Medicine 2075-4426 690468 http://zaguan.unizar.es/record/99058/files/texto_completo.pdf Versión publicada 2681213 http://zaguan.unizar.es/record/99058/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:99058 articulos driver 2023-05-18-14:25:25 ARTICLE