000099130 001__ 99130
000099130 005__ 20230519145448.0
000099130 0247_ $$2doi$$a10.1038/s42003-020-01536-6
000099130 0248_ $$2sideral$$a122581
000099130 037__ $$aART-2021-122581
000099130 041__ $$aeng
000099130 100__ $$aJiang, X.
000099130 245__ $$aExploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N2-fixation in plants
000099130 260__ $$c2021
000099130 5060_ $$aAccess copy available to the general public$$fUnrestricted
000099130 5203_ $$aEngineering nitrogen fixation in eukaryotes requires high expression of functional nitrogenase structural proteins, a goal that has not yet been achieved. Here we build a knowledge-based library containing 32 nitrogenase nifH sequences from prokaryotes of diverse ecological niches and metabolic features and combine with rapid screening in tobacco to identify superior NifH variants for plant mitochondria expression. Three NifH variants outperform in tobacco mitochondria and are further tested in yeast. Hydrogenobacter thermophilus (Aquificae) NifH is isolated in large quantities from yeast mitochondria and fulfills NifH protein requirements for efficient N2 fixation, including electron transfer for substrate reduction, P-cluster maturation, and FeMo-co biosynthesis. H. thermophilus NifH expressed in tobacco leaves shows lower nitrogenase activity than that from yeast. However, transfer of [Fe4S4] clusters from NifU to NifH in vitro increases 10-fold the activity of the tobacco-isolated NifH, revealing that plant mitochondria [Fe-S] cluster availability constitutes a bottleneck to engineer plant nitrogenases.
000099130 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E35-17R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64486-R
000099130 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000099130 590__ $$a6.548$$b2021
000099130 592__ $$a2.256$$b2021
000099130 594__ $$a6.0$$b2021
000099130 591__ $$aBIOLOGY$$b15 / 94 = 0.16$$c2021$$dQ1$$eT1
000099130 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2021$$dQ1
000099130 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2021$$dQ1
000099130 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000099130 700__ $$aPayá-Tormo, L.
000099130 700__ $$aCoroian, D.
000099130 700__ $$0(orcid)0000-0002-1827-1250$$aGarcía-Rubio, I.$$uUniversidad de Zaragoza
000099130 700__ $$aCastellanos-Rueda, R.
000099130 700__ $$aEseverri, Á.
000099130 700__ $$aLópez-Torrejón, G.
000099130 700__ $$aBurén, S.
000099130 700__ $$aRubio, L.M.
000099130 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000099130 773__ $$g4, 1 (2021), 4 [11 pp]$$tCommunications Biology$$x2399-3642
000099130 8564_ $$s562032$$uhttps://zaguan.unizar.es/record/99130/files/texto_completo.pdf$$yVersión publicada
000099130 8564_ $$s1560932$$uhttps://zaguan.unizar.es/record/99130/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000099130 909CO $$ooai:zaguan.unizar.es:99130$$particulos$$pdriver
000099130 951__ $$a2023-05-18-14:40:02
000099130 980__ $$aARTICLE