000099135 001__ 99135
000099135 005__ 20230519145448.0
000099135 0247_ $$2doi$$a10.3390/pathogens10020101
000099135 0248_ $$2sideral$$a122586
000099135 037__ $$aART-2021-122586
000099135 041__ $$aeng
000099135 100__ $$aGargano, V.
000099135 245__ $$aSerological survey and molecular characterization of theileria annulata in sicilian cattle
000099135 260__ $$c2021
000099135 5060_ $$aAccess copy available to the general public$$fUnrestricted
000099135 5203_ $$aTropical theileriosis is a tick-borne disease caused by hemoprotozoan parasites with considerable veterinary and economic impact worldwide. Ticks transmitting the disease belong to the Haemaphysalis, Rhipicephalus, and Hyalomma genera. The Hyalomma genus is very common in Sicily (Italy) and represents the main Theileria annulata vector in the island. Data concerning the molecular epidemiology of this pathogen are missing in the region. In 2018–2019, blood and serum samples were collected from 480 cows in seven Sicilian farms from four different provinces. Seroprevalence in the farms ranged from 22% to 71%. Three farms were selected for molecular analysis consisting of real-time PCR targeting the almost complete 18S ribosomal RNA (rRNA). Four amplicons per farm were sequenced and phylogenetic analyses were carried out. The four sequences were identical within each farm and showed 92–99% identity with the other farms and with sequences from Genbank. According to the phylogenetic analysis, these three sequences and an additional one from a laboratory-cultured Theileria annulata strain obtained in 1999 belonged to a single T. annulata clade with good bootstrap support with other sequences from Italy, India, and Iran, indicating limited geographical and temporal genetic variability of the parasite. This study represents the first phylogenetic analysis of T. annulata in Sicily, which will be useful to improve the strategies for theileriosis control and prevention.
000099135 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000099135 590__ $$a4.531$$b2021
000099135 592__ $$a0.901$$b2021
000099135 594__ $$a3.5$$b2021
000099135 591__ $$aMICROBIOLOGY$$b58 / 138 = 0.42$$c2021$$dQ2$$eT2
000099135 593__ $$aImmunology and Allergy$$c2021$$dQ2
000099135 593__ $$aMolecular Biology$$c2021$$dQ2
000099135 593__ $$aMicrobiology (medical)$$c2021$$dQ2
000099135 593__ $$aImmunology and Microbiology (miscellaneous)$$c2021$$dQ2
000099135 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000099135 700__ $$aBlanda, V.
000099135 700__ $$aGambino, D.
000099135 700__ $$aRussa, F.L.
000099135 700__ $$aDi Cataldo, S.
000099135 700__ $$aGentile, A.
000099135 700__ $$aSchirò, G.
000099135 700__ $$aTorina, A.
000099135 700__ $$0(orcid)0000-0001-5608-781X$$aMillán, J.
000099135 700__ $$aVicari, D.
000099135 773__ $$g10, 2 (2021), 101 [7 pp]$$pPathogens$$tPathogens$$x2076-0817
000099135 8564_ $$s207455$$uhttps://zaguan.unizar.es/record/99135/files/texto_completo.pdf$$yVersión publicada
000099135 8564_ $$s2853773$$uhttps://zaguan.unizar.es/record/99135/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000099135 909CO $$ooai:zaguan.unizar.es:99135$$particulos$$pdriver
000099135 951__ $$a2023-05-18-14:40:47
000099135 980__ $$aARTICLE