000099179 001__ 99179
000099179 005__ 20230519145403.0
000099179 0247_ $$2doi$$a10.3390/pathogens10010083
000099179 0248_ $$2sideral$$a122679
000099179 037__ $$aART-2021-122679
000099179 041__ $$aeng
000099179 100__ $$aRamírez, H.
000099179 245__ $$aAccurate diagnosis of small ruminant lentivirus infection is needed for selection of resistant sheep through tmem154 e35k genotyping
000099179 260__ $$c2021
000099179 5060_ $$aAccess copy available to the general public$$fUnrestricted
000099179 5203_ $$aSmall ruminant lentiviruses (SRLV) cause an incurable multiorganic disease widely spread in sheep and goats that disturbs animal welfare and production. In the absence of a vaccine, control measures have been traditionally based on early diagnosis and breeding with virus-inactivated colostrum with segregation of seropositive animals. However, antigenic heterogeneity, poor antibody production due to low viral load, and single strain design of most available ELISA, pose a threat to SRLV diagnosis. Genome-wide association studies have described TMEM154 E35K polymorphism as a good genetic marker for selection of resistant animals in some American and European breeds. In this study, a multitargeted serological and virological screening of more than 500 animals from four different breeds (latxa, raza Navarra, assaf, and churra) attending to SRLV infection status was performed. Then, animals were genotyped to characterize TMEM154 E35K polymorphism. ELISA procedures, individually considered, only identified a proportion of the seropositive animals, and PCR detected a fraction of seronegative animals, globally offering different animal classifications according to SRLV infection status. TMEM154 allele frequency differed substantially among breeds and a positive association between seroprevalence and TMEM154 genotype was found only in one breed. Selection based on TMEM154 may be suitable for specific ovine breeds or SRLV strains, however generalization to the whole SRLV genetic spectrum, ovine breeds, or epidemiological situation may need further validation.
000099179 536__ $$9info:eu-repo/grantAgreement/ES/MCIU/RTI2018-096172-B-C31
000099179 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000099179 590__ $$a4.531$$b2021
000099179 592__ $$a0.901$$b2021
000099179 594__ $$a3.5$$b2021
000099179 591__ $$aMICROBIOLOGY$$b58 / 138 = 0.42$$c2021$$dQ2$$eT2
000099179 593__ $$aImmunology and Allergy$$c2021$$dQ2
000099179 593__ $$aMolecular Biology$$c2021$$dQ2
000099179 593__ $$aMicrobiology (medical)$$c2021$$dQ2
000099179 593__ $$aImmunology and Microbiology (miscellaneous)$$c2021$$dQ2
000099179 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000099179 700__ $$aEcheverría, I.
000099179 700__ $$0(orcid)0000-0002-2497-9028$$aBenito, A.A.
000099179 700__ $$aGlaria, I.
000099179 700__ $$aBenavides, J.
000099179 700__ $$aPérez, V.
000099179 700__ $$ade Andrés, D.
000099179 700__ $$aReina, R.
000099179 773__ $$g10, 1 (2021), 83 [16 pp]$$pPathogens$$tPathogens$$x2076-0817
000099179 8564_ $$s763059$$uhttps://zaguan.unizar.es/record/99179/files/texto_completo.pdf$$yVersión publicada
000099179 8564_ $$s2706929$$uhttps://zaguan.unizar.es/record/99179/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000099179 909CO $$ooai:zaguan.unizar.es:99179$$particulos$$pdriver
000099179 951__ $$a2023-05-18-13:42:57
000099179 980__ $$aARTICLE