99254 20230519145432.0 doi 10.3389/fmicb.2021.623108 sideral 122575 ART-2021-122575 eng Mama, OM Prevalence and genetic characteristics of Staphylococcus aureus CC398 isolates from invasive infections in spanish hospitals, focusing on the livestock-independent CC398-MSSA clade 2021 Access copy available to the general public Unrestricted Background: Livestock-associated (LA)-CC398-MRSA is closely related to pigs, being unfrequently detected in human invasive infections. CC398-MSSA is emerging in human invasive infections in some countries, but genetic and epidemiological characteristics are still scarcely reported. Objectives: To determine the prevalence of Staphylococcus aureus (SA) CC398, both MRSA and MSSA, among blood cultures SA isolates recovered in Spanish hospitals located in regions with different pig-farming densities (PD) and characterize the recovered isolates. Methods: One thousand twenty-two SA isolates (761 MSSA, 261 MRSA) recovered from blood cultures during 6–12 months in 17 Spanish hospitals (2018–2019) were studied. CC398 lineage identification, detection of spa-types, and antibiotic resistance, virulence and human immune evasion cluster (IEC) genes were analyzed by PCR/sequencing. Results: Forty-four CC398-MSSA isolates (4.3% of SA; 5.8% of MSSA) and 10 CC398-MRSA isolates (1% of SA; 3.8% of MRSA) were detected. Eleven spa-types were found among the CC398-MSSA isolates with t571 and t1451 the most frequent spa-types detected (75%). Most of CC398-MSSA isolates were Immune-Evasion-Cluster (IEC)-positive (88.6%), tetracycline-susceptible (95.5%) and erythromycin/clindamycin–inducible-resistant/erm(T)-positive (75%). No statistical significance was detected when the CC398-MSSA/MSSA rate was correlated to PD (pigs/km2) (p = 0.108). On the contrary, CC398-MRSA isolates were all IEC-negative, predominately spa-t011 (70%), and the CC398-MRSA/MRSA rate was significantly associated to PD (p < 0.005). Conclusion: CC398-MSSA is an emerging clade in invasive infections in Spanish hospitals. CC398-MRSA (mostly t011) and CC398-MSSA (mostly t571 and t1451) show important differences, possibly suggesting divergent steps in host-adaptation evolutionary processes. While CC398-MRSA is livestock-associated (lacking IEC-system), CC398-MSSA seems to be mostly livestock-independent, carrying human-adaptation markers.. info:eu-repo/grantAgreement/ES/MINECO/SAF2016-76571-R info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 6.064 2021 MICROBIOLOGY 34 / 138 = 0.246 2021 Q1 T1 1.314 2021 Microbiology (medical) 2021 Q1 Microbiology 2021 Q1 8.2 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Aspiroz, C Ruiz-Ripa, L Ceballos, S Iñiguez-Barrio,M Cercenado, E Azcona, JM López-Cerero, L Seral, C Universidad de Zaragoza (orcid)0000-0002-9742-1463 López-Calleja, AI Belles-Belles, A Berdonces, P Siller, M Zarazaga, M Torres, C. Leiva, José Ezpeleta, Carmen Torroba,José Luis Castillo, Javier Universidad de Zaragoza (orcid)0000-0002-2519-701X Robres , Pilar Torres, Luis Alonso, Carla Andrea García, Mercé Navarro, María Vilamala, Anna Canut, Andrés Aguirre, Amaia Megías, Gregoria Ayarza, Rafael Gomáriz, María Calvo, Jorge Rezusta, Antonio Universidad de Zaragoza (orcid)0000-0001-7294-245X Palacian, Pilar 1011 630 Universidad de Zaragoza Dpto. Microb.Ped.Radio.Sal.Pú. Área Microbiología 12 (2021), 623108 [10 pp.] Front. microbiol. Frontiers in Microbiology 1664-302X 494471 http://zaguan.unizar.es/record/99254/files/texto_completo.pdf Versión publicada 2351407 http://zaguan.unizar.es/record/99254/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:99254 articulos driver 2023-05-18-14:19:13 ARTICLE