000099314 001__ 99314
000099314 005__ 20210223204725.0
000099314 0247_ $$2doi$$a10.1080/21505594.2019.1682762
000099314 0248_ $$2sideral$$a122831
000099314 037__ $$aART-2019-122831
000099314 041__ $$aeng
000099314 100__ $$aOuahrani-Bettache, S.
000099314 245__ $$aLethality of Brucella microti in a murine model of infection depends on the wbkE gene involved in O-polysaccharide synthesis
000099314 260__ $$c2019
000099314 5060_ $$aAccess copy available to the general public$$fUnrestricted
000099314 5203_ $$aBrucella microti was isolated a decade ago from wildlife and soil in Europe. Compared to the classical Brucella species, it exhibits atypical virulence properties such as increased growth in human and murine macrophages and lethality in experimentally infected mice. A spontaneous rough (R) mutant strain, derived from the smooth reference strain CCM4915T, showed increased macrophage colonization and was non-lethal in murine infections. Whole-genome sequencing and construction of an isogenic mutant of B. microti and Brucella suis 1330 revealed that the R-phenotype was due to a deletion in a single gene, namely wbkE (BMI_I539), encoding a putative glycosyltransferase involved in lipopolysaccharide (LPS) O-polysaccharide biosynthesis. Complementation of the R-strains with the wbkE gene restored the smooth phenotype and the ability of B. microti to kill infected mice. LPS with an intact O-polysaccharide is therefore essential for lethal B. microti infections in the murine model, demonstrating its importance in pathogenesis.
000099314 536__ $$9info:eu-repo/grantAgreement/ES/INIA/RTA2017-00083-00-00
000099314 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000099314 590__ $$a5.542$$b2019
000099314 591__ $$aIMMUNOLOGY$$b33 / 158 = 0.209$$c2019$$dQ1$$eT1
000099314 591__ $$aMICROBIOLOGY$$b22 / 134 = 0.164$$c2019$$dQ1$$eT1
000099314 591__ $$aINFECTIOUS DISEASES$$b9 / 93 = 0.097$$c2019$$dQ1$$eT1
000099314 592__ $$a1.757$$b2019
000099314 593__ $$aImmunology$$c2019$$dQ1
000099314 593__ $$aInfectious Diseases$$c2019$$dQ1
000099314 593__ $$aParasitology$$c2019$$dQ1
000099314 593__ $$aMicrobiology (medical)$$c2019$$dQ1
000099314 593__ $$aMicrobiology$$c2019$$dQ1
000099314 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000099314 700__ $$0(orcid)0000-0002-5490-5177$$aJiménez De Bagüés, M.P.
000099314 700__ $$aDe La Garza, J.
000099314 700__ $$aFreddi, L.
000099314 700__ $$aBueso, J.P.
000099314 700__ $$aLyonnais, S.
000099314 700__ $$aAl Dahouk, S.
000099314 700__ $$aDe Biase, D.
000099314 700__ $$aKöhler, S.
000099314 700__ $$aOcchialini, A.
000099314 773__ $$g10, 1 (2019), 868-878$$pVIRULENCE$$tVirulence$$x2150-5594
000099314 8564_ $$s2915095$$uhttps://zaguan.unizar.es/record/99314/files/texto_completo.pdf$$yVersión publicada
000099314 8564_ $$s1232020$$uhttps://zaguan.unizar.es/record/99314/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000099314 909CO $$ooai:zaguan.unizar.es:99314$$particulos$$pdriver
000099314 951__ $$a2021-02-23-19:03:52
000099314 980__ $$aARTICLE