000099323 001__ 99323
000099323 005__ 20210223204726.0
000099323 0247_ $$2doi$$a10.1088/2053-1591/ab6636
000099323 0248_ $$2sideral$$a122878
000099323 037__ $$aART-2019-122878
000099323 041__ $$aeng
000099323 100__ $$aEstevez, M.B.
000099323 245__ $$aBiogenic silver nanoparticles: Understanding the antimicrobial mechanism using Confocal Raman Microscopy
000099323 260__ $$c2019
000099323 5060_ $$aAccess copy available to the general public$$fUnrestricted
000099323 5203_ $$aThe antimicrobial properties of silver nanoparticles (AgNPs) have made them ubiquitous in a number of real-world industrial applications; however, the antimicrobial mode of action of biogenic AgNPs is not entirely understood. The use of Raman spectroscopy can provide molecular fingerprint information on various chemical and biochemical components in complex systems like microbial cultures, without the need for any complex sample pre-treatment. Consequently, the antimicrobial mechanism of AgNPs can be inferred through morphological and compositional changes of microbial cells that are monitored via changes in Raman band profiles. Here we show the synthesis of biogenic AgNPs using the extracellular cell-free filtrates of Penicillium expansum. The antimicrobial activity of the Penicillium expansum synthesized silver nanoparticles (hereafter PeNPs) was evaluated and the interactions between the nanoparticles and Escherichia coli were studied using Transmission Electron Microscopy (TEM) and Environmental Scanning Electron Microscopy (ESEM), showing the attachment of PeNPs to the surface of the bacteria and rupture of the bacterial cell membrane. Importantly, we show how Confocal Raman Microscopy can be used as an innovative approach to study the antimicrobial mechanisms, the results of which confirm that the PeNPs induce damage to bacterial and fungal cells, resulting in critical changes to polysaccharides, lipids, proteins and nucleic acids.
000099323 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000099323 590__ $$a1.929$$b2019
000099323 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b203 / 314 = 0.646$$c2019$$dQ3$$eT2
000099323 592__ $$a0.365$$b2019
000099323 593__ $$aMetals and Alloys$$c2019$$dQ2
000099323 593__ $$aPolymers and Plastics$$c2019$$dQ2
000099323 593__ $$aSurfaces, Coatings and Films$$c2019$$dQ2
000099323 593__ $$aElectronic, Optical and Magnetic Materials$$c2019$$dQ3
000099323 593__ $$aBiomaterials$$c2019$$dQ4
000099323 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000099323 700__ $$0(orcid)0000-0003-4848-414X$$aMitchell, S.G.$$uUniversidad de Zaragoza
000099323 700__ $$aFaccio, R.
000099323 700__ $$aAlborés, S.
000099323 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000099323 773__ $$g6, 12 (2019), 1250f5 [13 pp]$$pMat. res. express$$tMaterials Research Express$$x2053-1591
000099323 8564_ $$s1241801$$uhttps://zaguan.unizar.es/record/99323/files/texto_completo.pdf$$yPostprint
000099323 8564_ $$s1327653$$uhttps://zaguan.unizar.es/record/99323/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000099323 909CO $$ooai:zaguan.unizar.es:99323$$particulos$$pdriver
000099323 951__ $$a2021-02-23-19:04:27
000099323 980__ $$aARTICLE