99350 20230914083325.0 doi 10.21451/1984-3143-AR2018-0100 sideral 122886 ART-2019-122886 eng Domingo, P. Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C 2019 Access copy available to the general public Unrestricted Artificial insemination with cooled semen is the most common practice in rabbit farms and any improvement on it helps to increase the efficiency and productivity of rabbit meat farms. Therefore, the aim of this study was to assess whether different cryoprotectant agents (CPA) as glycerol, N, N-Dimethylformamide (DMF) and N-Methyl--2-Pyrrolidone (NMP) can improve cooled rabbit sperm quality stored at 4C and 16C. Sperm samples were diluted with INRA 96® (Extender A), INRA 96® with 6% glycerol (Extender B) or 6% DMF (Extender C) or 6% NMP (Extender D) respectively and stored at 4C and 16C. Samples were then analysed at 4, 24, 48 and 72 hours after refrigeration by integrated sperm analysis system (ISAS), eosin-nigrosin stain (vitality), hypo-osmotic swelling test (HOS test) and acrosome integrity test. Extender C showed higher percentage of motility, vitality and HOS test than extender B and D (p<0.05). Whereas sperm quality decreased over time (p<0.05), data showed that the addition of DMF kept the motility and sperm plasma membrane integrity after 24 hours of storage better than other diluents. These results suggest that the addition of DMF to INRA 96® exerts a protective effect on the membrane of spermatozoa improving seminal quality. info:eu-repo/grantAgreement/ES/DGA-FSE/Aragon Research Groups info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 0.916 2019 AGRICULTURE, DAIRY & ANIMAL SCIENCE 41 / 63 = 0.651 2019 Q3 T2 0.355 2019 Veterinary (miscellaneous) 2019 Q2 Animal Science and Zoology 2019 Q3 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Olaciregui, M. (orcid)0000-0001-6479-3933 González, N. Universidad de Zaragoza (orcid)0000-0002-1611-6337 De Blas, I. Universidad de Zaragoza (orcid)0000-0002-1204-4356 Gil, L. Universidad de Zaragoza (orcid)0000-0002-0797-1841 1009 773 Universidad de Zaragoza Dpto. Patología Animal Área Sanidad Animal 1009 617 Universidad de Zaragoza Dpto. Patología Animal Área Medicina y Cirugía Animal 16, 4 (2019), 887-894 Anim. reprod. Animal Reproduction 1806-9614 726706 http://zaguan.unizar.es/record/99350/files/texto_completo.pdf Versión publicada 3155782 http://zaguan.unizar.es/record/99350/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:99350 articulos driver 2023-09-13-11:08:28 ARTICLE