99360 20210902121633.0 doi 10.1016/j.colsurfb.2020.110904 sideral 117159 ART-2020-117159 eng Ortiz de Solorzano, Isabel (orcid)0000-0002-6256-742X Customized hybrid and NIR-light triggered thermoresponsive drug delivery microparticles synthetized by photopolymerization in a one-step flow focusing continuous microreactor 2020 Access copy available to the general public Unrestricted Photopolymerization is a selective technique that takes advantage of light-sensitive molecules to initiate and propagate monomeric structures to render covalently bonded macromolecular materials structures known as polymers. Herein, we present a novel one-step microfluidic synthesis of customized hybrid-thermoresponsive Poly(N-isopropylacrylamide) (PNIPAm) based microparticles (MPs) containing plasmonic hollow gold nanoparticles (HGNPs) and bupivacaine (BVP) used as a model drug. Those hybrid microparticles were prepared using a flow-focusing microreactor coupled to a UV LED device built with a simple outer PTFE tubing and an inner flexible capillary. Different tubing characteristics and flow rate ratios were altered in order to control the size of the resulting microparticles. In addition, components such as monomer, crosslinker and photoinitiator concentrations, as well as LED intensity and irradiation time were tuned to obtain different MPs and their characteristics and polymerization rates were compared by Gel Permeation Chromatography (GPC). Thermoresponsive properties were analyzed and the presence of HGNPs was confirmed in light-activated triggered drug release applications. Bupivacaine loading and release studies were evaluated with the resulting hollow and solid microparticles (which were obtained depending on the polymerization rate used) and their temperature responsiveness was assessed using a NIR laser when HGNPs were present in the constructs. Finally, cytotoxicity studies, cell-cycle arrest and apoptotic induction were carried out to certify their suitability for further biomedical applications to be used as triggerable drug depots. info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM info:eu-repo/semantics/openAccess by-nc-nd http://creativecommons.org/licenses/by-nc-nd/3.0/es/ 5.268 2020 BIOPHYSICS 11 / 71 = 0.155 2020 Q1 T1 MATERIALS SCIENCE, BIOMATERIALS 15 / 40 = 0.375 2020 Q2 T2 CHEMISTRY, PHYSICAL 54 / 162 = 0.333 2020 Q2 T2 0.938 2020 Biotechnology 2020 Q1 Colloid and Surface Chemistry 2020 Q1 Surfaces and Interfaces 2020 Q1 Physical and Theoretical Chemistry 2020 Q1 Medicine (miscellaneous) 2020 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Mendoza, Gracia Universidad de Zaragoza (orcid)0000-0003-2293-363X Arruebo, Manuel Universidad de Zaragoza (orcid)0000-0003-3165-0156 Sebastian, Víctor Universidad de Zaragoza (orcid)0000-0002-6873-5244 5005 555 Universidad de Zaragoza Dpto. Ing.Quím.Tecnol.Med.Amb. Área Ingeniería Química 190 (2020), 110904 1-11 Colloids surf., B Biointerfaces COLLOIDS AND SURFACES B-BIOINTERFACES 0927-7765 554801 http://zaguan.unizar.es/record/99360/files/texto_completo.pdf Postprint 980404 http://zaguan.unizar.es/record/99360/files/texto_completo.jpg?subformat=icon icon Postprint oai:zaguan.unizar.es:99360 articulos driver 2021-09-02-08:54:46 ARTICLE