99444 20231006143304.0 doi 10.22489/CinC.2020.303 sideral 123039 ART-2020-123039 eng Celotto, Chiara Universidad de Zaragoza Relationship between Atrial Oscillatory Acetylcholine Release Pattern and f-wave Frequency Modulation: a Computational and Experimental Study 2020 Access copy available to the general public Unrestricted The frequency of fibrillatory waves (f-waves), F f , exhibits significant variation over time, and previous studies suggest that some of this variation is related to respiratory modulation through the autonomic nervous system. In this study, we tested the hypothesis that this variation (ΔF f ) could be related to acetylcholine concentration ([ACh]) release pattern. Electrocardiograms were recorded from seven patients during controlled respiration before and after full vagal blockade, from which f-wave frequency modulation was characterized. Computational simulations in human atrial tissues were performed to assess the effects of [ACh] release pattern on F f and compared to experimental results in humans. A cross-stimulation protocol was applied onto the tissue to initiate a rotor while cyclically varying [ACh] following a sinusoidal waveform of frequency equal to 0.125 Hz. Different mean levels (0.05, 0.075μM/l) and peak-to-peak ranges (0.1, 0.05, 0.025 μM/l) of [ACh] variation were tested. In all patients, an f-wave frequency modulation could be observed. In 57% of the patients, this modulation was significantly reduced after vagal blockade. Simulations confirmed that rotor frequency variations followed the induced [ACh] patterns. Mean F f was dependent on mean [ACh] level, whileΔF f was dependent on [ACh] variation range. info:eu-repo/grantAgreement/ES/DGA-FSE/T39-20R-BSICoS group info:eu-repo/grantAgreement/ES/DGA/LMP124-18 info:eu-repo/grantAgreement/EUR/ERC-2014-StG-638284 info:eu-repo/grantAgreement/EC/H2020/766082/EU/MultidisciplinarY training network for ATrial fibRillation monItoring, treAtment and progression/MY-ATRIA This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 766082-MY-ATRIA info:eu-repo/grantAgreement/ES/MICINN/PID2019-104881RB-I00 info:eu-repo/grantAgreement/ES/MINECO/PID2019-105674RB-I00 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 0.257 2020 Computer Science (miscellaneous) 2020 Cardiology and Cardiovascular Medicine 2020 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Sánchez Tapia, Carlos (orcid)0000-0003-4273-5403 Mountris, Konstantinos (orcid)0000-0003-2946-3044 Abdollahpur, Mostafa Sandberg, Frida Laguna Lasaosa, Pablo Universidad de Zaragoza (orcid)0000-0003-3434-9254 Pueyo Paúles, Esther Universidad de Zaragoza (orcid)0000-0002-1960-407X 5008 800 Universidad de Zaragoza Dpto. Ingeniería Electrón.Com. Área Teoría Señal y Comunicac. 47, ID303 (2020), [4 pp.] Comput. cardiol. Computing in Cardiology 2325-8861 1373303 http://zaguan.unizar.es/record/99444/files/texto_completo.pdf Versión publicada 2654366 http://zaguan.unizar.es/record/99444/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:99444 articulos driver 2023-10-06-14:07:16 ARTICLE