000099698 001__ 99698 000099698 005__ 20230519145419.0 000099698 0247_ $$2doi$$a10.1038/s41598-021-83421-8 000099698 0248_ $$2sideral$$a123138 000099698 037__ $$aART-2021-123138 000099698 041__ $$aeng 000099698 100__ $$aVan Eeckhout, A. 000099698 245__ $$aPolarimetric imaging microscopy for advanced inspection of vegetal tissues 000099698 260__ $$c2021 000099698 5060_ $$aAccess copy available to the general public$$fUnrestricted 000099698 5203_ $$aOptical microscopy techniques for plant inspection benefit from the fact that at least one of the multiple properties of light (intensity, phase, wavelength, polarization) may be modified by vegetal tissues. Paradoxically, polarimetric microscopy although being a mature technique in biophotonics, is not so commonly used in botany. Importantly, only specific polarimetric observables, as birefringence or dichroism, have some presence in botany studies, and other relevant metrics, as those based on depolarization, are underused. We present a versatile method, based on a representative selection of polarimetric observables, to obtain and to analyse images of plants which bring significant information about their structure and/or the spatial organization of their constituents (cells, organelles, among other structures). We provide a thorough analysis of polarimetric microscopy images of sections of plant leaves which are compared with those obtained by other commonly used microscopy techniques in plant biology. Our results show the interest of polarimetric microscopy for plant inspection, as it is non-destructive technique, highly competitive in economical and time consumption, and providing advantages compared to standard non-polarizing techniques. 000099698 536__ $$9info:eu-repo/grantAgreement/ES/MCIU-FEDER/RTI2018-097107-B-C31 000099698 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/ 000099698 590__ $$a4.997$$b2021 000099698 592__ $$a1.005$$b2021 000099698 591__ $$aMULTIDISCIPLINARY SCIENCES$$b19 / 74 = 0.257$$c2021$$dQ2$$eT1 000099698 593__ $$aMultidisciplinary$$c2021$$dQ1 000099698 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000099698 700__ $$aGarcia-Caurel, E. 000099698 700__ $$aGarnatje, T. 000099698 700__ $$aEscalera, J.C. 000099698 700__ $$aDurfort, M. 000099698 700__ $$aVidal, J. 000099698 700__ $$0(orcid)0000-0003-1740-2244$$aGil, J.J.$$uUniversidad de Zaragoza 000099698 700__ $$aCampos, J. 000099698 700__ $$aLizana, A. 000099698 7102_ $$12002$$2385$$aUniversidad de Zaragoza$$bDpto. Física Aplicada$$cÁrea Física Aplicada 000099698 773__ $$g11, 1 (2021), 3913 [12 pp]$$pSci. rep. (Nat. Publ. Group)$$tScientific reports (Nature Publishing Group)$$x2045-2322 000099698 8564_ $$s646789$$uhttps://zaguan.unizar.es/record/99698/files/texto_completo.pdf$$yVersión publicada 000099698 8564_ $$s2834464$$uhttps://zaguan.unizar.es/record/99698/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000099698 909CO $$ooai:zaguan.unizar.es:99698$$particulos$$pdriver 000099698 951__ $$a2023-05-18-14:05:08 000099698 980__ $$aARTICLE