99698 20230519145419.0 doi 10.1038/s41598-021-83421-8 sideral 123138 ART-2021-123138 eng Van Eeckhout, A. Polarimetric imaging microscopy for advanced inspection of vegetal tissues 2021 Access copy available to the general public Unrestricted Optical microscopy techniques for plant inspection benefit from the fact that at least one of the multiple properties of light (intensity, phase, wavelength, polarization) may be modified by vegetal tissues. Paradoxically, polarimetric microscopy although being a mature technique in biophotonics, is not so commonly used in botany. Importantly, only specific polarimetric observables, as birefringence or dichroism, have some presence in botany studies, and other relevant metrics, as those based on depolarization, are underused. We present a versatile method, based on a representative selection of polarimetric observables, to obtain and to analyse images of plants which bring significant information about their structure and/or the spatial organization of their constituents (cells, organelles, among other structures). We provide a thorough analysis of polarimetric microscopy images of sections of plant leaves which are compared with those obtained by other commonly used microscopy techniques in plant biology. Our results show the interest of polarimetric microscopy for plant inspection, as it is non-destructive technique, highly competitive in economical and time consumption, and providing advantages compared to standard non-polarizing techniques. info:eu-repo/grantAgreement/ES/MCIU-FEDER/RTI2018-097107-B-C31 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 4.997 2021 MULTIDISCIPLINARY SCIENCES 19 / 74 = 0.257 2021 Q2 T1 1.005 2021 Multidisciplinary 2021 Q1 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Garcia-Caurel, E. Garnatje, T. Escalera, J.C. Durfort, M. Vidal, J. Gil, J.J. Universidad de Zaragoza (orcid)0000-0003-1740-2244 Campos, J. Lizana, A. 2002 385 Universidad de Zaragoza Dpto. Física Aplicada Área Física Aplicada 11, 1 (2021), 3913 [12 pp] Sci. rep. (Nat. Publ. Group) Scientific reports (Nature Publishing Group) 2045-2322 646789 http://zaguan.unizar.es/record/99698/files/texto_completo.pdf Versión publicada 2834464 http://zaguan.unizar.es/record/99698/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:99698 articulos driver 2023-05-18-14:05:08 ARTICLE