99754 20230519145400.0 doi 10.3390/v13010093 sideral 123274 ART-2021-123274 eng Cañada, J.E. Identification of a New HIV-1 BC Intersubtype Circulating Recombinant Form (CRF108_BC) in Spain 2021 Access copy available to the general public Unrestricted The extraordinary genetic variability of human immunodeficiency virus type 1 (HIV-1) group M has led to the identification of 10 subtypes, 102 circulating recombinant forms (CRFs) and numerous unique recombinant forms. Among CRFs, 11 derived from subtypes B and C have been identified in China, Brazil, and Italy. Here we identify a new HIV-1 CRF_BC in Northern Spain. Originally, a phylogenetic cluster of 15 viruses of subtype C in protease-reverse transcriptase was identified in an HIV-1 molecular surveillance study in Spain, most of them from individuals from the Basque Country and heterosexually transmitted. Analyses of near full-length genome sequences from six viruses from three cities revealed that they were BC recombinant with coincident mosaic structures different from known CRFs. This allowed the definition of a new HIV-1 CRF designated CRF108_BC, whose genome is predominantly of subtype C, with four short subtype B fragments. Phylogenetic analyses with database sequences supported a Brazilian ancestry of the parental subtype C strain. Coalescent Bayesian analyses estimated the most recent common ancestor of CRF108_BC in the city of Vitoria, Basque Country, around 2000. CRF108_BC is the first CRF_BC identified in Spain and the second in Europe, after CRF60_BC, both phylogenetically related to Brazilian subtype C strains. info:eu-repo/grantAgreement/ES/ISCIII-AESI/PI16CIII-00033 info:eu-repo/grantAgreement/ES/ISCIII-AESI/PI19CIII-00042 info:eu-repo/grantAgreement/ES/ISCIII-RIS-FEDER/RD16ISCIII-0002-0004 info:eu-repo/semantics/openAccess by http://creativecommons.org/licenses/by/3.0/es/ 5.818 2021 VIROLOGY 14 / 37 = 0.378 2021 Q2 T2 1.463 2021 Virology 2021 Q1 Infectious Diseases 2021 Q1 6.6 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Delgado, E. Gil, H. Sánchez, M. Benito, S. García-Bodas, E. Gómez-González, C. Canut-Blasco, A. Portu-Zapirain, J. Sáez de Adana, E. De la Peña, M. Ibarra, S. Cilla, G. Iribarren, J.A. Martínez-Sapiña, A. Thomson, M.M. 13, 1 (2021), 93 [13 pp] VIRUSES-BASEL VIRUSES-BASEL 1999-4915 863301 http://zaguan.unizar.es/record/99754/files/texto_completo.pdf Versión publicada 2713703 http://zaguan.unizar.es/record/99754/files/texto_completo.jpg?subformat=icon icon Versión publicada oai:zaguan.unizar.es:99754 articulos driver 2023-05-18-13:37:06 ARTICLE