Gold nanoparticle formation as an indicator of enzymatic methods: colorimetric L-phenylalanine determination

Martín-Barreiro, Alba ; de Marcos, Susana (Universidad de Zaragoza) ; Galbán, Javier (Universidad de Zaragoza)

Resumen: An enzymatic-colorimetric method has been developed based on the reaction between l-phenylalanine (l-Phe) and the l-amino acid oxidase (LAAO) in the presence of Au(III), which has led to the formation of gold nanoparticles. The intensity of the localized surface plasmon resonance (LSPR) band of the generated nanoparticles (550 nm) can be related to the concentration of l-Phe in the sample. The mechanism of the LAAO-l-Phe enzyme reaction in the presence of Au(III) has been studied through the evaluation and optimization of experimental conditions. These studies have reinforced the hypothesis that the catalytic center of the enzyme helps the Au(III) reduction and, thanks to the protein, the Au0 form is stabilized as gold nanoparticles (AuNPs). In the calibration study, a sigmoidal relationship between the concentration of the substrate and the LSPR of the nanoparticles was observed. The linearization of the signal has allowed the determination of l-Phe in the range from 17 to 500 µM with an RSD% (150 μM) of 4.8% (n = 3). The method is free of other amino acid interference normally found in blood plasma. These highly competitive results open the possibility of further development of a rapid method for l-Phe determination based on colorimetry.

Idioma: Inglés

DOI: 10.1007/s00216-022-03900-3

Año: 2022

Publicado en: Analytical and Bioanalytical Chemistry 414, 8 (2022), 2641-2649
ISSN: 1618-2642

Factor impacto JCR: 4.3 (2022)

Categ. JCR: CHEMISTRY, ANALYTICAL rank: 20 / 86 = 0.233 (2022) - Q1 - T1
Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 18 / 77 = 0.234 (2022) - Q1 - T1

Factor impacto CITESCORE: 7.5 - Chemistry (Q1) - Biochemistry, Genetics and Molecular Biology (Q2)

Factor impacto SCIMAGO: 0.676 - Biochemistry (Q2) - Analytical Chemistry (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E25-20R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)