RNAi-based glyconanoparticles trigger apoptotic pathways for in vitro and in vivo enhanced cancer-cell killing

Conde, J. ; Tian, F. ; Hernandez, Y. ; Bao, C. ; Baptista, P.V. ; Cui, D. ; Stoeger, T. ; de la Fuente, J.M. (Universidad de Zaragoza)

Resumen: Gold glyconanoparticles (GlycoNPs) are full of promise in areas like biomedicine, biotechnology and materials science due to their amazing physical, chemical and biological properties. Here, siRNA GlycoNPs (AuNP@PEG@Glucose@siRNA) in comparison with PEGylated GlycoNPs (AuNP@PEG@Glucose) were applied in vitro to a luciferase-CMT/167 adenocarcinoma cancer cell line and in vivo via intratracheal instillation directly into the lungs of B6 albino mice grafted with luciferase-CMT/167 adenocarcinoma cells. siRNA GlycoNPs but not PEGylated GlycoNPs induced the expression of pro-apoptotic proteins such as Fas/CD95 and caspases 3 and 9 in CMT/167 adenocarcinoma cells in a dose dependent manner, independent of the inflammatory response, evaluated by bronchoalveolar lavage cell counting. Moreover, in vivo pulmonary delivered siRNA GlycoNPs were capable of targeting c-Myc gene expression (a crucial regulator of cell proliferation and apoptosis) via in vivo RNAi in tumour tissue, leading to an ~80% reduction in tumour size without associated inflammation.

Idioma: Inglés

DOI: 10.1039/c4nr05742b

Año: 2015

Publicado en: Nanoscale 7, 19 (2015), 9083-9091
ISSN: 2040-3364

Factor impacto JCR: 7.76 (2015)

Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 23 / 271 = 0.085 (2015) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 12 / 83 = 0.145 (2015) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 18 / 163 = 0.11 (2015) - Q1 - T1
Categ. JCR: PHYSICS, APPLIED rank: 12 / 145 = 0.083 (2015) - Q1 - T1

Factor impacto SCIMAGO: 2.77 - Nanoscience and Nanotechnology (Q1) - Materials Science (miscellaneous) (Q1)

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)