Efficiency of Antimicrobial Electrospun Thymol-Loaded Polycaprolactone Mats in Vivo

García-Salinas, S. (Universidad de Zaragoza) ; Gámez, E. ; Asín, J. ; De Miguel, R. (Universidad de Zaragoza) ; Andreu, V. ; Sancho-Albero, M. (Universidad de Zaragoza) ; Mendoza, G. (Universidad de Zaragoza) ; Irusta, S. (Universidad de Zaragoza) ; Arruebo, M. (Universidad de Zaragoza)

Resumen: Due to the prevalence of antimicrobial resistant pathogens, natural products with long-term antimicrobial activities are considered as potential alternatives. In this work, polycaprolactone (PCL) electrospun fibers with mean diameters around 299 nm and loaded with 14.92 ± 1.31% w/w thymol (THY) were synthesized. The mats had appropriate elongation at break (74.4 ± 9.5%) and tensile strength (3.0 ± 0.5 MPa) to be potentially used as wound dressing materials. In vivo studies were performed using eight to ten week-old male SKH1 hairless mice. The infection progression was evaluated through a semiquantitative method and quantitative polymerase chain reaction. The analyses of post-mortem samples indicated that THY-loaded PCL fibers acted as inhibitors of Staphylococcus aureus ATCC 25923 strain growth being as efficient as chlorhexidine (CLXD). Histopathological and immunohistochemical studies showed that the PCL-THY-treated wounds were almost free of an inflammatory reaction. Therefore, wound dressings containing natural compounds can prevent infection and promote wound healing and prompt regeneration. Copyright

Idioma: Inglés

DOI: 10.1021/acsabm.0c00419

Año: 2020

Publicado en: ACS Applied Bio Materials 3, 5 (2020), 3430-3439
ISSN: 2576-6422

Factor impacto SCIMAGO: 0.764 - Biochemistry (medical) (Q1) - Chemistry (miscellaneous) (Q1) - Biomedical Engineering (Q1) - Biomaterials (Q1)

Financiación: info:eu-repo/grantAgreement/EUR/ERC-2013-CoG-614715
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2014-52384-R

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Área (Departamento): Área Tecnologi. Medio Ambiente (Dpto. Ing.Quím.Tecnol.Med.Amb.)