000101536 001__ 101536
000101536 005__ 20220405150421.0
000101536 0247_ $$2doi$$a10.1002/cctc.201902404
000101536 0248_ $$2sideral$$a117956
000101536 037__ $$aART-2020-117956
000101536 041__ $$aeng
000101536 100__ $$0(orcid)0000-0001-9478-6750$$aVelasco-Lozano, S.
000101536 245__ $$aCo-immobilization and Colocalization of Multi-Enzyme Systems for the Cell-Free Biosynthesis of Aminoalcohols
000101536 260__ $$c2020
000101536 5060_ $$aAccess copy available to the general public$$fUnrestricted
000101536 5203_ $$aThe manufacturing of aminoalcohols is an appealing transformation for industrial biocatalysis due to their high value as building blocks in synthetic chemistry. By co-immobilizing dehydrogenases, ¿-transaminases and oxidases on porous carriers, we fabricated and characterized a multi-functional heterogeneous biocatalyst that was further applied to the cell-free biosynthesis of amines and aminoalcohols from alcohols and diols, respectively. This immobilized cascade integrates both redox cofactor and amine donor recycling systems into the amino alcohol biosynthesis. Spatial co-localization of the multi-enzyme system significantly increases the cofactor recycling efficiency, the system productivity and the product conversion. Using this multi-functional heterogeneous biocatalyst, we achieved 80 % conversion of alcohols into amines and accessed a palette of up to 5 aminoalcohols starting from their corresponding diols in one-pot. The results herein presented contribute to understanding the effects of the confinement of co-immobilized enzymes on the cascade processes.
000101536 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E37-17R$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón$$9info:eu-repo/grantAgreement/EC/Era-CoBiotech/HOMBIOCAT/PCI2018-092984$$9info:eu-repo/grantAgreement/ES/MICINN/MDM-2017-0720$$9info:eu-repo/grantAgreement/ES/MINECO/BIO2015-69887-R
000101536 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000101536 590__ $$a5.686$$b2020
000101536 591__ $$aCHEMISTRY, PHYSICAL$$b50 / 162 = 0.309$$c2020$$dQ2$$eT1
000101536 592__ $$a1.497$$b2020
000101536 593__ $$aCatalysis$$c2020$$dQ1
000101536 593__ $$aPhysical and Theoretical Chemistry$$c2020$$dQ1
000101536 593__ $$aOrganic Chemistry$$c2020$$dQ1
000101536 593__ $$aInorganic Chemistry$$c2020$$dQ1
000101536 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000101536 700__ $$aSantiago-Arcos, J.
000101536 700__ $$0(orcid)0000-0003-1570-4257$$aMayoral, J.A.$$uUniversidad de Zaragoza
000101536 700__ $$aLópez-Gallego, F.
000101536 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000101536 773__ $$g12, 11 (2020), 3030-3041$$pChemCatChem$$tChemCatChem$$x1867-3880
000101536 8564_ $$s1053207$$uhttps://zaguan.unizar.es/record/101536/files/texto_completo.pdf$$yPostprint
000101536 8564_ $$s1099002$$uhttps://zaguan.unizar.es/record/101536/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000101536 909CO $$ooai:zaguan.unizar.es:101536$$particulos$$pdriver
000101536 951__ $$a2022-04-05-14:40:10
000101536 980__ $$aARTICLE