Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2

Santiago, Llipsy ; Uranga-Murillo, Iratxe ; Arias, Maykel ; González-Ramírez, Andrés M. (Universidad de Zaragoza) ; Macías-León, Javier (Universidad de Zaragoza) ; Moreo, Eduardo (Universidad de Zaragoza) ; Redrado, Sergio ; García-García, Ana (Universidad de Zaragoza) ; Taleb, Víctor (Universidad de Zaragoza) ; Lira-Navarrete, Erandi (Universidad de Zaragoza) ; Hurtado-Guerrero, Ramón ; Aguilo, Nacho (Universidad de Zaragoza) ; del Mar Encabo-Berzosa, Maria ; Hidalgo, Sandra ; Galvez, Eva M. ; Ramirez-Labrada, Ariel ; de Miguel, Diego ; Benito, Rafael (Universidad de Zaragoza) ; Miranda, Patricia ; Fernández, Antonio (Universidad de Zaragoza) ; Domingo, José M. ; Serrano, Laura ; Yuste, Cristina ; Villanueva-Saz, Sergio (Universidad de Zaragoza) ; Paño-Pardo, José R. (Universidad de Zaragoza) ; Pardo, Julián (Universidad de Zaragoza)
Determination of the Concentration of IgG against the Spike Receptor-Binding Domain That Predicts the Viral Neutralizing Activity of Convalescent Plasma and Serum against SARS-CoV-2
Resumen: Several hundred millions of people have been diagnosed of coronavirus disease 2019 (COVID-19), causing millions of deaths and a high socioeconomic burden. SARS-CoV-2, the causative agent of COVID-19, induces both specific T- and B-cell responses, being antibodies against the virus detected a few days after infection. Passive immunization with hyperimmune plasma from convalescent patients has been proposed as a potentially useful treatment for COVID-19. Using an in-house quantitative ELISA test, we found that plasma from 177 convalescent donors contained IgG antibodies specific to the spike receptor-binding domain (RBD) of SARS-CoV-2, although at very different concentrations which correlated with previous disease severity and gender. Anti-RBD IgG plasma concentrations significantly correlated with the plasma viral neutralizing activity (VN) against SARS-CoV-2 in vitro. Similar results were found using an independent cohort of serum from 168 convalescent health workers. These results validate an in-house RBD IgG ELISA test in a large cohort of COVID-19 convalescent patients and indicate that plasma from all convalescent donors does not contain a high enough amount of anti-SARS-CoV-2-RBD neutralizing IgG to prevent SARS-CoV-2 infection in vitro. The use of quantitative anti-RBD IgG detection systems might help to predict the efficacy of the passive immunization using plasma from patients recovered from SARS-CoV-2.
Idioma: Inglés
DOI: 10.3390/biology10030208
Año: 2021
Publicado en: Biology 10, 3 (2021), 208 [14 pp.]
ISSN: 2079-7737

Factor impacto JCR: 5.168 (2021)
Categ. JCR: BIOLOGY rank: 21 / 94 = 0.223 (2021) - Q1 - T1
Factor impacto CITESCORE: 2.8 - Agricultural and Biological Sciences (Q2) - Immunology and Microbiology (Q3) - Biochemistry, Genetics and Molecular Biology (Q3)

Factor impacto SCIMAGO: 0.903 - Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q1) - Agricultural and Biological Sciences (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/B29-17R
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/E34-R17
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP58-18
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/COV20-00308
Financiación: info:eu-repo/grantAgreement/ES/MCIU-AEI/SAF2017-83120-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2016-75633-P
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2013-44367-C2-2-P
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2019-105451GB-I00
Tipo y forma: Article (Published version)
Área (Departamento): Área Microbiología (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Area Medicina (Dpto. Medicina, Psiqu. y Derm.)
Área (Departamento): Área Inmunología (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)


Creative Commons You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.


Exportado de SIDERAL (2023-11-08-20:14:28)


Visitas y descargas

Este artículo se encuentra en las siguientes colecciones:
Articles



 Record created 2021-05-07, last modified 2023-11-08


Versión publicada:
 PDF
Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)