Regulation by FurC in Anabaena links the oxidative stress response to photosynthetic metabolism

Sevilla, Emma (Universidad de Zaragoza) ; Sarasa-Buisán, Cristina (Universidad de Zaragoza) ; González, Andrés (Universidad de Zaragoza) ; Cases, Rafael (Universidad de Zaragoza) ; Kufryk, Galyna ; Peleato, M. Luisa (Universidad de Zaragoza) ; Fillat, María F. (Universidad de Zaragoza)

Resumen: The FUR (Ferric Uptake Regulator) family in Anabaena sp. PCC 7120 consists of three paralogs named FurA (Fur), FurB (Zur) and FurC (PerR). furC seems to be an essential gene in the filamentous nitrogen-fixing strain Anabaena sp. PCC 7120, suggesting that it plays a fundamental role in this organism. In order to better understand the functions of FurC in Anabaena, the phenotype of a derivative strain that overexpresses this regulator (EB2770FurC) has been characterized. The furC-overexpressing variant presented alterations in growth rate, morphology and ultrastructure, as well as higher sensitivity to peroxide than Anabaena sp. PCC 7120. Interestingly, the overexpression of furC led to reduced photosynthetic O2 evolution, increased respiratory activity, and had a significant influence in the composition and efficiency of both photosystems. Comparative transcriptional analyses, together with electrophoretic mobility shift assays allowed the identification of different genes directly controlled by FurC, and involved in processes not previously related to PerR proteins, such as the cell division gene ftsZ and the major thylakoid membrane protease ftsH. The rise in the transcription of ftsH in EB2770FurC cells correlated with reduced levels of the D1 protein, which is involved in the PSII repair cycle. Deregulation of the oxidative stress response in EB2770FurC cells led to the identification of novel FurC targets involved in the response to H2O2 through different mechanisms. These results, together with the effect of furC overexpression on the composition, stability and efficiency of the photosynthetic machinery of Anabaena, disclose novel links between PerR proteins, cell division and photosynthesis in filamentous cyanobacteria.

Idioma: Inglés

DOI: 10.1093/pcp/pcz094

Año: 2019

Publicado en: Plant & Cell Physiology 60, 8 (2019), 1778–1789
ISSN: 1471-9053

Factor impacto JCR: 4.062 (2019)

Categ. JCR: PLANT SCIENCES rank: 25 / 234 = 0.107 (2019) - Q1 - T1
Categ. JCR: CELL BIOLOGY rank: 81 / 194 = 0.418 (2019) - Q2 - T2

Factor impacto SCIMAGO: 2.021 - Cell Biology (Q1) - Plant Science (Q1) - Physiology (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E35-17R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2012-31458
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P

Tipo y forma: Article (PrePrint)

Área (Departamento): Área Fisiología Vegetal (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Física Materia Condensada (Dpto. Física Materia Condensa.)