Identification of free-living amoebas and amoeba-resistant bacteria accumulated in Dreissena polymorpha

Goñi, Pilar (Universidad de Zaragoza) ; Benito, María ; La Plante, Daniella ; Fernández, María T. (Universidad de Zaragoza) ; Sánchez, Elena ; Chueca, Patricia (Universidad de Zaragoza) ; Miguel, Natividad (Universidad de Zaragoza) ; Mosteo, Rosa (Universidad de Zaragoza) ; Ormad, María P. (Universidad de Zaragoza) ; Rubio, Encarnación (Universidad de Zaragoza)

Resumen: To identify the free-living amoeba (FLA) and amoeba-resistant bacteria (ARB) accumulated in zebra mussels and in the water in which they are found, mussels were collected at two locations in the Ebro river basin (North East Spain). FLAs and bacteria were isolated from mussel extracts and from natural water. PCR techniques were used to identify the FLAs and endosymbiont bacteria (Legionella, Mycobacterium, Pseudomonas and cyanobacteria), and to detect Giardia and Cryptosporidium. The most frequently found FLAs were Naegleria spp. The presence of Legionella, Mycobacterium and Pseudomonas inside the FLA was demonstrated, and in some cases both Legionella and Pseudomonas were found together. Differences between FLAs and ARB identified inside the mussels and in the water were detected. In addition, Escherichia coli, Clostridium perfringens, Salmonella spp. and Enterococcus spp. were accumulated in mussels in concentrations unconnected with those found in water. The results show the ability of the zebra mussel to act as a reservoir of potentially pathogenic FLAs, which are associated with potentially pathogenic ARB, although the lack of association between microorganisms inside the mussels and in the water suggests that they are not useful for monitoring microbiological contamination at a specific time.

Idioma: Inglés

DOI: 10.1111/1462-2920.15093

Año: 2020

Publicado en: Environmental Microbiology 22, 8 (2020), 3315-3324
ISSN: 1462-2912

Factor impacto JCR: 5.491 (2020)

Categ. JCR: MICROBIOLOGY rank: 30 / 136 = 0.221 (2020) - Q1 - T1

Factor impacto SCIMAGO: 1.954 - Microbiology (Q1) - Ecology, Evolution, Behavior and Systematics (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/B43-20R
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Parasitología (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Área Enfermería (Dpto. Fisiatría y Enfermería)
Área (Departamento): Área Técnica. Lab. y Talleres (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Área Medic.Prevent.Salud Públ. (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Área Tecnologi. Medio Ambiente (Dpto. Ing.Quím.Tecnol.Med.Amb.)