Priming maritime pine megagametophytes during somatic embryogenesis improved plant adaptation to heat stress

Pérez-Oliver, M.A. ; Haro, J.G. ; Pavlovic, I. ; Novák, O. ; Segura, J. ; Sales, E. (Universidad de Zaragoza) ; Arrillaga, I.

Resumen: In the context of global climate change, forest tree research should be addressed to provide genotypes with increased resilience to high temperature events. These improved plants can be obtained by heat priming during somatic embryogenesis (SE), which would produce an epigenetic-mediated transgenerational memory. Thereby, we applied 37 °C or 50 °C to maritime pine (Pinus pinaster) megagametophytes and the obtained embryogenic masses went through the subsequent SE phases to produce plants that were further subjected to heat stress conditions. A putative transcription factor WRKY11 was upregulated in priming-derived embryonal masses, and also in the regenerated P37 and P50 plants, suggesting its role in establishing an epigenetic memory in this plant species. In vitro-grown P50 plants also showed higher cytokinin content and SOD upregula-tion, which points to a better responsiveness to heat stress. Heat exposure of two-year-old maritime pine plants induced upregulation of HSP70 in those derived from primed embryogenic masses, that also showed better osmotic adjustment and higher increases in chlorophyll, soluble sugars and starch contents. Moreover, ¿PSII of P50 plants was less affected by heat exposure. Thus, our results suggest that priming at 50 °C at the SE induction phase is a promising strategy to improve heat resilience in maritime pine.

Idioma: Inglés

DOI: 10.3390/plants10030446

Año: 2021

Publicado en: Plants 10, 3 (2021), 446 [24 pp.]
ISSN: 2223-7747

Factor impacto JCR: 4.658 (2021)

Categ. JCR: PLANT SCIENCES rank: 39 / 240 = 0.162 (2021) - Q1 - T1

Factor impacto CITESCORE: 3.6 - Environmental Science (Q2) - Agricultural and Biological Sciences (Q2)

Factor impacto SCIMAGO: 0.765 - Plant Science (Q1) - Ecology, Evolution, Behavior and Systematics (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MICINN-FEDER/AGL2016-76143-C4-01-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Producción Vegetal (Dpto. CC.Agrar.y Medio Natural)