Enhanced piezoelectric fibered extracellular matrix to promote cardiomyocyte maturation and tissue formation: a 3d computational model

Urdeitx, P. (Universidad de Zaragoza) ; Doweidar, M.H. (Universidad de Zaragoza)

Resumen: Mechanical and electrical stimuli play a key role in tissue formation, guiding cell processes such as cell migration, differentiation, maturation, and apoptosis. Monitoring and controlling these stimuli on in vitro experiments is not straightforward due to the coupling of these different stimuli. In addition, active and reciprocal cell–cell and cell–extracellular matrix interactions are essential to be considered during formation of complex tissue such as myocardial tissue. In this sense, computational models can offer new perspectives and key information on the cell microenvironment. Thus, we present a new computational 3D model, based on the Finite Element Method, where a complex extracellular matrix with piezoelectric properties interacts with cardiac muscle cells during the first steps of tissue formation. This model includes collective behavior and cell processes such as cell migration, maturation, differentiation, proliferation, and apoptosis. The model has employed to study the initial stages of in vitro cardiac aggregate formation, considering cell–cell junctions, under different extracellular matrix configurations. Three different cases have been purposed to evaluate cell behavior in fibered, mechanically stimulated fibered, and mechanically stimulated piezoelectric fibered extra-cellular matrix. In this last case, the cells are guided by the coupling of mechanical and electrical stimuli. Accordingly, the obtained results show the formation of more elongated groups and enhancement in cell proliferation.

Idioma: Inglés

DOI: 10.3390/biology10020135

Año: 2021

Publicado en: Biology 10, 2 (2021), 135 [27 pp.]
ISSN: 2079-7737

Factor impacto JCR: 5.168 (2021)

Categ. JCR: BIOLOGY rank: 21 / 94 = 0.223 (2021) - Q1 - T1

Factor impacto CITESCORE: 2.8 - Agricultural and Biological Sciences (Q2) - Immunology and Microbiology (Q3) - Biochemistry, Genetics and Molecular Biology (Q3)

Factor impacto SCIMAGO: 0.903 - Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q1) - Agricultural and Biological Sciences (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/T24-20R
Financiación: info:eu-repo/grantAgreement/ES/MINECO-AEI/PID2019-104009RB-I00-AEI-10.13039-501100011033

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)