000106588 001__ 106588
000106588 005__ 20210902121927.0
000106588 0247_ $$2doi$$a10.1021/acs.nanolett.0c02163
000106588 0248_ $$2sideral$$a120307
000106588 037__ $$aART-2020-120307
000106588 041__ $$aeng
000106588 100__ $$0(orcid)0000-0001-7625-4806$$aPiñol, R.$$uUniversidad de Zaragoza
000106588 245__ $$aReal-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles
000106588 260__ $$c2020
000106588 5060_ $$aAccess copy available to the general public$$fUnrestricted
000106588 5203_ $$aMeasurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ~20 degrees and subcellular gradients of ~5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
000106588 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E11-17R$$9info:eu-repo/grantAgreement/EC/H2020/801305/EU/Nanoparticles-based 2D thermal bioimaging technologies/NanoTBTech$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 801305-NanoTBTech$$9info:eu-repo/grantAgreement/EC/H2020/829162/EU/Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation/HOTZYMES$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 829162-HOTZYMES$$9info:eu-repo/grantAgreement/ES/MINECO-FEDER/PGC2018-095795-B-I00
000106588 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000106588 590__ $$a11.189$$b2020
000106588 591__ $$aCHEMISTRY, PHYSICAL$$b22 / 162 = 0.136$$c2020$$dQ1$$eT1
000106588 591__ $$aMATERIALS SCIENCE, MULTIDISCIPLINARY$$b32 / 333 = 0.096$$c2020$$dQ1$$eT1
000106588 591__ $$aNANOSCIENCE & NANOTECHNOLOGY$$b15 / 106 = 0.142$$c2020$$dQ1$$eT1
000106588 591__ $$aPHYSICS, CONDENSED MATTER$$b11 / 69 = 0.159$$c2020$$dQ1$$eT1
000106588 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b20 / 178 = 0.112$$c2020$$dQ1$$eT1
000106588 591__ $$aPHYSICS, APPLIED$$b15 / 160 = 0.094$$c2020$$dQ1$$eT1
000106588 592__ $$a4.852$$b2020
000106588 593__ $$aBioengineering$$c2020$$dQ1
000106588 593__ $$aChemistry (miscellaneous)$$c2020$$dQ1
000106588 593__ $$aNanoscience and Nanotechnology$$c2020$$dQ1
000106588 593__ $$aMaterials Science (miscellaneous)$$c2020$$dQ1
000106588 593__ $$aMechanical Engineering$$c2020$$dQ1
000106588 593__ $$aCondensed Matter Physics$$c2020$$dQ1
000106588 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000106588 700__ $$aZeler, J.
000106588 700__ $$aBrites, C.D.S.
000106588 700__ $$aGu, Y.
000106588 700__ $$0(orcid)0000-0002-1819-4785$$aTéllez, P.$$uUniversidad de Zaragoza
000106588 700__ $$aCarneiro Neto, A.N.
000106588 700__ $$ada Silva, T.E.
000106588 700__ $$0(orcid)0000-0002-6600-1618$$aMoreno-Loshuertos, R.$$uUniversidad de Zaragoza
000106588 700__ $$0(orcid)0000-0001-8971-7355$$aFernandez-Silva, P.$$uUniversidad de Zaragoza
000106588 700__ $$aGallego, A.I.
000106588 700__ $$0(orcid)0000-0003-3043-147X$$aMartinez-Lostao, L.$$uUniversidad de Zaragoza
000106588 700__ $$0(orcid)0000-0002-8797-0813$$aMartínez, A.$$uUniversidad de Zaragoza
000106588 700__ $$aCarlos, L.D.
000106588 700__ $$0(orcid)0000-0003-0828-3212$$aMillán, A.$$uUniversidad de Zaragoza
000106588 7102_ $$15008$$2785$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Tecnología Electrónica
000106588 7102_ $$11011$$2566$$aUniversidad de Zaragoza$$bDpto. Microb.Ped.Radio.Sal.Pú.$$cÁrea Inmunología
000106588 7102_ $$10$$2X$$aUniversidad de Zaragoza$$bServ.Gral. Apoyo Investigación$$cDivisión: Serv. Transversales
000106588 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000106588 7102_ $$12003$$2395$$aUniversidad de Zaragoza$$bDpto. Física Materia Condensa.$$cÁrea Física Materia Condensada
000106588 773__ $$g20, 9 (2020), 6466-6472$$pNano lett.$$tNano Letters$$x1530-6984
000106588 8564_ $$s3119560$$uhttps://zaguan.unizar.es/record/106588/files/texto_completo.pdf$$yPostprint
000106588 8564_ $$s1814284$$uhttps://zaguan.unizar.es/record/106588/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000106588 909CO $$ooai:zaguan.unizar.es:106588$$particulos$$pdriver
000106588 951__ $$a2021-09-02-10:52:30
000106588 980__ $$aARTICLE