Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles

Piñol, R. (Universidad de Zaragoza) ; Zeler, J. ; Brites, C.D.S. ; Gu, Y. ; Téllez, P. (Universidad de Zaragoza) ; Carneiro Neto, A.N. ; da Silva, T.E. ; Moreno-Loshuertos, R. (Universidad de Zaragoza) ; Fernandez-Silva, P. (Universidad de Zaragoza) ; Gallego, A.I. ; Martinez-Lostao, L. (Universidad de Zaragoza) ; Martínez, A. (Universidad de Zaragoza) ; Carlos, L.D. ; Millán, A. (Universidad de Zaragoza)

Resumen: Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ~20 degrees and subcellular gradients of ~5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.

Idioma: Inglés

DOI: 10.1021/acs.nanolett.0c02163

Año: 2020

Publicado en: Nano Letters 20, 9 (2020), 6466-6472
ISSN: 1530-6984

Factor impacto JCR: 11.189 (2020)

Categ. JCR: CHEMISTRY, PHYSICAL rank: 22 / 162 = 0.136 (2020) - Q1 - T1
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 32 / 333 = 0.096 (2020) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 15 / 106 = 0.142 (2020) - Q1 - T1
Categ. JCR: PHYSICS, CONDENSED MATTER rank: 11 / 69 = 0.159 (2020) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 20 / 178 = 0.112 (2020) - Q1 - T1
Categ. JCR: PHYSICS, APPLIED rank: 15 / 160 = 0.094 (2020) - Q1 - T1

Factor impacto SCIMAGO: 4.852 - Bioengineering (Q1) - Chemistry (miscellaneous) (Q1) - Nanoscience and Nanotechnology (Q1) - Materials Science (miscellaneous) (Q1) - Mechanical Engineering (Q1) - Condensed Matter Physics (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E11-17R
Financiación: info:eu-repo/grantAgreement/EC/H2020/801305/EU/Nanoparticles-based 2D thermal bioimaging technologies/NanoTBTech
Financiación: info:eu-repo/grantAgreement/EC/H2020/829162/EU/Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation/HOTZYMES
Financiación: info:eu-repo/grantAgreement/ES/MINECO-FEDER/PGC2018-095795-B-I00

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Tecnología Electrónica (Dpto. Ingeniería Electrón.Com.)
Área (Departamento): Área Inmunología (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): División: Serv. Transversales (Serv.Gral. Apoyo Investigación)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Física Materia Condensada (Dpto. Física Materia Condensa.)